胃癌肿瘤引流淋巴结内Foxp3^+调节性T细胞的分布

Distribution of Foxp3^+ regulatory T cells in tumor-draining lymph nodes of human gastric cancer

采用免疫组织化学方法检测胃癌肿瘤引流淋巴结和第二站淋巴结内调节性T细胞(Treg细胞)的分布和细胞数量。结果显示Foxp3+Treg细胞弥散地分布于胃癌引流淋巴结的副皮质区和髓质区。胃癌肿瘤引流淋巴结内Treg细胞数明显多于第二站淋巴结(P<0.01),进展期胃癌肿瘤引流淋巴结内Treg细胞数多于早期胃癌(P<0.01)。Treg细胞聚集胃癌肿瘤引流淋巴结内可能通过形成免疫耐受环境而促进肿瘤淋巴转移的发生。

To compare the number and distribution of Foxp3 ＋ regulatory T cells （Treg） in tumor-draining lymph nodes（TDLN, N1） and N2 to assess whether Treg may contribute to the lymphatic metastasis in human gastric cancer. Foxp3 ＋ regulatory T ceils in N1 and N2 were detected by immunohistochemical staining. Foxp3＋ Treg showed diffuse distribution in paracortex and medulla. TDLN tended to be enriched in Foxp3 ＋ Treg compared with N2 （P 〈0. 01 ）. Foxp3 ＋ regulatory T cells in TDLN from advanced gastric cancer were significantly higher than that from early gastric cancer （P 〈 0. 01 ）. Foxp3 ＋ regulatory T cells exhibit increased recruitment to TDLN, which could facilitate the immune tolerance and contribute to lymphatic metastasis of tumor cells.