非小细胞肺癌组织中性粒细胞明胶酶相关蛋白表达及其功能研究

Expression and function of neutrophil gelatinase associated lipocalin protein in non - small cell lung carcinomas

目的探讨中性粒细胞明胶酶相关蛋白（NGAL蛋白）在在非小细胞肺癌（NSCLC）组织的表达及其对NSCLC患者的临床意义。方法收集我院60例原发性NSCLC手术切除的癌组织石蜡标本作为研究组，30例正常组织标本作为对照组，采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（sP）法检测NGAL蛋白表达，比较组间表达差异，分析临床、病理因素对NGAL表达的影响。结果60例NSCLC标本NGAL阳性率为68．3％，高于正常组织的阳性率（13．3％,X2=24．200，P=0．000），腺癌NGAL阳性率（65．7％）与鳞癌阳性率（72．0％）差异无统计学意义（X2=0．266，P=0．606）；随着TNM分期增加，NGAL阳性率升高（X2=11．682，P：0．001）；淋巴结转移组NGAL阳性率（80．9％）显著高于无淋巴结转移组（23．1％，X2=15．708，P=0．000）；肿瘤直径〉3cm组NGAL阳性率（80．0％）显著高于≤3cm组（45．0％，X2=7．548，P=0．006）。结论NGAL蛋白在NSCLC组织中呈高表达，与NSCLC的发生、进展和转移明显相关。

Objective To study the expression of neutrophil gelatinase associated lipocalin （NGAL） protein in non - small cell lung carcinomas and its clinical significance. Methods Cancer tissue paraffin specimens of 60 primary non - small - cell lung carcinoma （ NSCLC ） patients were collected as study group, and 60 normal tissue araffin specimens were collected as control group. The expression of NGAL protein was detected by mmunohistochemistry （SP） , and compared between two groups. The influ- ence of clinical pathology factors on the expression of NGAL was analyzed. Results NSCLC positive rate in NSCLC group （68.3%） was higher than in control group （ 13.3% ） （X2 =24. 200,P =0. 000）. There was no significant difference in positive rate between squamous carcinoma group and adenocarcinoma group （X2 =0.266, P= 0.606）. NGAL positive rate was increased as TNM increased （X2 = 11.682, P= 0. 001 ）. NGAL positive rate in metastasis group （80. 9% ） was higher than in non - metastasis group （X2 = 15. 708, P = 0. 000）. NGAL positive rate in 〉 3 em tumor diameter group （80. 9% ） was higher than in ≤ 3 cm tumor diameter group （X2 = 7. 548, P = 0. 006）. Conclusion NGAL protein was over expressed in NSCLC tissue, and was correlated with occurrence, development and metastasis of cancer.