不同浓度姜黄素后处理对原代心肌细胞缺氧／复氧损伤的保护作用

The protective effect of curcumin post - processing with different concentrations on primary myo- cardial cell during hypoxia/ reoxygenation injury

目的 制备原代心肌细胞缺氧/复氧（H/R）模型，探讨姜黄素后处理对H/R损伤保护作用的最佳浓度。 方法 实验分为5组：正常对照组；H/R组；姜黄素不同浓度（1、5、10 μmol/L）＋H/R组。通过噻唑蓝（MTT）法检测细胞活性；检测培养液中乳酸脱氢酶（LDH）活性、丙二醛（MDA）浓度和超氧化物歧化酶（SOD）活性，评估细胞损伤；通过原位缺口末端标记法（TUNEL）法检测细胞凋亡率。 结果 与对照组比较，H/R组细胞活性降低（P＝0.001），各浓度姜黄素组显著增强细胞活性，尤以10 μmol/L组效果最好（P＝0.017）；与H/R组比较，各浓度姜黄素组显著降低LDH活性，显著降低MDA浓度，显著提高SOD活性，明显改善H/R损伤后的心肌细胞凋亡率，其中均以10 μmol/L组效果最明显（P＝0.011、0.001、0.009、0.007），且与对照组比较差异无统计学意义（P＝0.129、0.083、0.134）。 结论 在造模成功的基础上，不同浓度姜黄素后处理均具有保护心肌细胞损伤，抗氧化应激，抗凋亡的作用，尤以10 μmol/L效果最好，为最佳药物浓度。

Objective To establish the original generation of myocardial cells in vitro anoxia/reoxygenation （H/R） model, then to explore the optimum concentration of curcumin post-processing on protection of primary Myocardial cell during hypoxia/reoxygenation （H/R） Injury. Methods The experimental group was divided into 5 groups： normal control group; H/R group; Curcumin concentrations （1, 5, 10 μmol/L）＋ H/R group. Through the cell activity determined by methyl thiazol tetrazolium （MTT） method and test lactate dehydrogenase （LDH） activity and malondialdehyde （MDA） concentration and superoxide dismutase （SOD） activity evaluation of cell damage; To detect apoptosis rate by TdT-mediated dUTP nick end labeling （TUNEL） method. Results Compared with control group, cell activity decrease in H/R group （P=0.001）, the curcumin group significantly enhanced the activity of cells, especially in the 10 μmol/L group （P=0.017）; Compared with control group, the curcumin group of different concentrations significantly decreased the activity of LDH, decreased MDA concentration, increased SOD activity and improve the myocardial cell apoptosis after H/R damage rate significantly. Among them, the effect of 10 μmol/L group was the most obvious （P=0.011, 0.001, 0.009, 0.007）, and there was no significant difference compared with the control group （P=0.129, 0.083, 0.134）. Conclusion On the basis of the success of the model, curcumin of different concentrations have protective effects on myocardial cells, anti oxidative stress and anti apoptosis, the concentration of 10 μmol/L is optimum.