基于断层扫描的兔坐骨神经显微结构三维可视化研究

Three - dimensional reconstruction of rabbit sciatic nerve bundles based on micro - computed tomography

目的 探讨采用显微断层扫描技术（Micro-CT）采集新西兰大白兔坐骨神经断层数据，通过Mimics三维可视化软件实现兔坐骨神经内部显微结构的三维可视化。方法 取新西兰大白兔坐骨神经组织标本，A、B组分别用1%、5% Lugol’s液对其染色，显微镜下观察记录分别A、B组在0.5、1.0、1.5、2.0、2.5、3.0、3.5 h时间点上神经染色情况，通过Micro-CT采集A、B组神经断层数据，观察选择其中合适的染色参数。4个标本采用Mimics 17.0软件重建兔坐骨神经神经显微立体结构。结果 A组兔坐骨神经标本在染色2.5 h可获得较为真实、清晰的显微三维结构显示效果。Mimics软件观察测量提示新西兰大白兔坐骨神经主要分3组神经束，重建模型测量横截面面积平均分别为0.425、0.038、0.242 mm2。可在任意断面放大观察坐骨神经显微结构，追踪3组神经束立体行径相对固定。结论 基于断层扫描的兔坐骨神经显微结构三维可视化系统能较清晰、真实地反映兔坐骨神经内部结构，可为周围神经虚拟三维重建技术提供新方法及可行性依据。

Objective To reconstruct the three-dimensional microstructure of the New Zealand rabbit sciatic nerve bundle by Micro-CT.Methods Sciatic nerve tissue were harvested from New Zealand rabbits and placed into 1% （Group A）, 5% （Group B） Lugol’s solution respectively, The 2 Groups were evaluated under microscope and Micro-CT at different time 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 h. Nerve data from Micro-CT were imported to the Mimics software to calculated 3D digital model.Results The conditions for sciatic nerve have a better display quality under Micro-CT were 1% concentration and 2.5 h staining time. The sciatic nerve of New Zealand rabbits divides into 3 bundles and the average cross-sectional area were 0.425, 0.038, 0.242 mm2. The pathways of 3 nerve bundles were relatively fixed, no obviously crossing and mergers shown.Conclusion The sciatic nerve of New Zealand rabbits stained by Lugol’s solution can reflect the internal structure clearly by Micro-CT scanning. And these finding may provide a new method to observe three-dimensional construction of peripheral nerves.