艾灸“肺俞、膏肓俞”对BLMA5所致肺纤维化大鼠肺上皮细胞E-cad基因表达的实验研究

Effects of Moxibustion of Feishu( BL13) and Gaohuangshu( BL43) on Expression of E-cad Gene in Lung Epithelial Cells in BLMA5-induced Pulmonary Fibrosis Rats

目的:探究艾灸"肺俞、膏肓俞"抑制肺纤维化进程的作用机制。方法 :将81只8周龄SPF级大鼠随机分9组:空白对照组;模型组(7 d组、11 d组、23 d组、35 d组);艾灸组(11 d组、23 d组、35 d组);阳性对照(泼尼松)组,每组9只。采用气管内一次性注入博来霉素复制肺纤维化模型。各组造模均于第二天进行治疗及干预。艾灸组交替艾灸双侧肺俞和膏育俞,3壮/穴,5 mg/壮,1次/d,10次/疗程。泼尼松组以泼尼松灌胃。空白组和所有模型组与艾灸组采取同样的抓取和固定,不给予任何治疗。共治疗3个疗程,疗程间休息2 d。各组在设定的时间窗口处死动物,采用定量PCR法检测E-cad基因的表达水平。结果:艾灸治疗组E-cad基因表达量比同期模型组高,尤以第35 d明显。艾灸35 d组(0.32±0.07)明显高于模型35 d组(0.10±0.04)(P<0.01)。艾灸组和泼尼松组无明显差异,统计学无意义(P>0.05)。结论:艾灸"肺俞、膏肓俞"可抑制肺纤维化的进程,其内在机制可能与升高肺上皮细胞E-cad基因表达有关。

Objective： To investigate the mechanism of moxibustion at Feishu（ BL13） and Gaohuang（ BL43） on bleomycin-induced pulmonary fibrosis. Methods： Eighty-one healthy male SD rates were randomly divided into nine groups： blank control group,model group（ 7 d group,11 d group,23 d group,35 d group）,moxibustion group（ 11 d group,23 d group,35 d group）,positive control group（ prednisone group）（ n = 9）. The exprimental pulmonary fibrosis rat model was induced by a single injection of the bleomycin via trachea. Treatment and intervention were carried out on second day. Moxibustion group was alternatively given moxibustion at bilateral Feishu（ BL13） and Gaohuang（ BL43）,3 Zhuang/point,5 mg/Zhuang,once a day,10 times a course of treatment. Prednisone group was treated with prednisone orally. But blank control group did not give any treatment. There was a total of 3 courses and 2 days rest between the course of treatment. The animals were sacrificed at the time window and the expression level of E-cad gene was detected by quantitative PCR method. Results： The expression of E-cad in the treatment group was higher than that in the model group in the same period,especially on the thirty-fifth day. Moxibustion 35 d group（ 0. 32 ± 0. 07）was significantly higher than that of model 35 d group（ 0. 10 ± 0. 04）（ P〈0. 01）. Moxibustion group and prednisone group had no significant difference（ P〈0. 05）. Conclusion： Moxibustion at Feishu（ BL13） and Gaohuang（ BL43） can inhibit the progression of pulmonary fibrosis and the underlying mechanism may be related to the increased expression of E-cad gene in lung epithelial cells.