莱克多巴胺新型分子印迹纳米管膜的研究及应用

Novel Molecularly Imprinted Polymer Nanotube Membranes for Ractopamine

基于表面引发原子转移自由基聚合(ATRP),采用阳极氧化铝(AAO)膜合成了莱克多巴胺印迹聚合物(MIP)纳米管膜,以甲基丙烯酸(MAA)作为功能单体,n(莱克多巴胺)∶n(MAA)为1∶6.利用扫描电子显微镜(SEM)对MIP纳米管膜的形态进行表征,结果显示,在AAO表面成功修饰了MIP纳米管膜.与莱克多巴胺NIP纳米管膜相比,MIP纳米管膜对莱克多巴胺及其类似物有更高的吸附容量和更好的选择性.建立了MIP纳米管膜萃取与HPLC联用,针对β_2-肾上腺素受体激动剂检测的方法.莱克多巴胺(RAC)的线性范围是10~1 000μg/L,克伦特罗(CLEN)、肾上腺素(EP)和多巴胺(DA)的线性范围为100~1 000μg/L,特布他林(TER)的线性范围是200~1 000μg/L.检出限的范围在0.74~2.50μg/L.该方法能对猪肉样品中β_2-肾上腺素受体激动剂实现有效检测.

A method for the synthesis of ractopamine molecularly imprinted polymer （MIP） nanotube membranes using an anodic alumina oxide （AAO） template by surface-initiated atom transfer radical polymerization （ATRP） was presented.Methacrylic acid （MAA） was selected as functional monomer and the polymerization rate of n（ractopamine）∶n（MAA） was 1∶6.The morphology of MIP nanotube membranes were characterized by scanning electron microscope （SEM）.The SEM results showed that ATRP route works well in the formation of MIP nanotubes within AAO template.A series of adsorption experiments revealed that the MIP nanotube membranes showed better extraction capacity and good selectivity than that of non-imprinted polymer （NIP） nanotube membranes for ractopamine and its analogues.In order to evaluate the usability of the MIP nanotube membranes, a methodology by combining MIP nanotube membranes extraction coupled with high performance liquid chromatography （HPLC） detection for the determination of β2-agonists in complex samples was developed.The linear ranges were 10~1 000 μg/L for ractopamine, 100~1 000 μg/L for clenbuterol, epinephrine and dopamine, and 200~1 000 μg/L for terbutaline.The detection limits were within the range of 0.74~2.50 μg/L.The proposed method is suitable for the determination of trace β2-agonists in pork samples.