铝毒胁迫下水稻根冠黏液中铝形态的变化

Change of Aluminum Speciation in Rice Root Cap Mucilage Under Aluminum Toxicity Stress

以耐铝性有明显差异的2个水稻品种Ⅱ优3027(耐铝基因型)和红良优166(铝敏感基因型)为材料,研究了不同铝浓度下根冠黏液、根尖(悬空培养、振荡培养)中总铝、单核铝含量以及铝形态变化。结果表明,在铝胁迫下,振荡培养组中2个品种总铝、单核铝含量随着铝浓度的上升而上升;悬空培养组根尖和黏液中,Ⅱ优3027总铝、单核铝含量在0~200μmol/L铝浓度范围时随着铝浓度的上升而上升,在200~400μmol/L铝浓度范围时,呈下降趋势,而红良优166变化规律与振荡培养组基本一致。耐铝品种和敏感品种在0~200μmol/L铝浓度范围时铝含量相近,但在400μmol/L铝处理浓度时差异显著。振荡培养和悬空培养条件下铝形态变化规律也大致同上,并发现对水稻起毒害作用的铝形态主要是交换态铝和吸附态铝,有机态铝毒性很小。

Two rice varieties ＇ Ⅱ you 3027＇ （patient）and ＇Hongliangyou 166＇ （sensitive）with obvious difference in aluminium sensitivity were used as test material to study the change of total aluminium,monocyte aluminium content and form at different aluminum concentration.The results showed that two rice varieties＇ total aluminium, monocyte aluminium content was increased with aluminum concentrations under oscillation cultivation and the same to＇ Hongliangyou 166＇ under submerged cultivation. ＇ Ⅱ you 3027＇ total aluminium,monocyte aluminium content was increased with aluminum concentrations in 0-200μmol/L and declined with aluminum concentrations in 200 ~400μmol/L under submerged cultivation. The aluminum content of the resistant aluminum varieties and sensitive varieties is similar in the range of 0~200μmol/L aluminum concentration,but the aluminum treatment concentration was significantly different in 400 μmol/L aluminum concentration. The main forms of aluminum toxicity to rice were exchangeable aluminum and adsorbed aluminum ,while organic aluminum is very toxic.