EGCG对肾癌细胞786-O增殖及血管化的影响

Effects of EGCG on the proliferation and angiogenesis of 786-O cell of renal carcinoma

目的探讨表没食子儿茶素没食子酸酯(EGCG)对肾癌细胞增殖以及血管生成的作用。方法将不同浓度(0、10、30、60μmol/L)的EGCG与肾癌786-O细胞作用后,采用MTT法检测EGCG对肾癌细胞增殖能力以及通过ELISA法检测EGCG对肾癌细胞分泌血管内皮生长因子(VEGF)能力的影响;通过建立裸鼠肾癌动物模型,以不同剂量(0、30、100 mg/kg)经口途径喂服EGCG,测量肾癌肿瘤生长情况并绘制肿瘤生长曲线,使用RT-PCR检测肿瘤组织中VEGF mRNA表达,使用免疫组织化学方法测定肾癌组织中微血管的生成。结果 EGCG能够抑制786-O细胞的增殖能力,其抑制能力随其浓度的升高和作用时间的延长而增强,呈现浓度和时间依赖性(P<0.05)。同时EGCG还能以浓度依赖的方式抑制786-O细胞分泌VEGF的能力(P<0.05)。进一步研究发现EGCG能够抑制786-O细胞体内的生长,同时降低786-O肿瘤组织中VEGF mRNA的表达水平以及新生微血管的面积(P<0.05)。结论 EGCG具有抗肾癌的作用,能够抑制肾癌的生长以及血管的生成。

Objective To investigate the effect of epigallocatechin-3-gallate（EGCG）on the proliferation and angiogenesis of renal cancer cells. Methods Cancer 786-O cells were treated with different concentrations of EGCG,then the cell viability was assessed using an MTT assay and the concentration of VEGF in 786-O cells culture media was examined by ELISA assay.Cancer 786-O cells were subcutaneously injected in BALB/c nude mice,the mice were randomly divided into three groups and EGCG was administered orally every day at 0,30 and 100mg/kg body weight to group A,B,C respectively.Tumor tissues were measured every other day and the tumor growth curve was drawn.RT-PCR was used to evaluate the expression level of VEGF mRNA in tumor tissues,and we examined the microvessel density in tumor tissues by immunohistochemical analysis. ResultsEGCG inhibited cell proliferation in a concentration-and time-dependent manner（P〈0.05）.EGCG inhibited cell releasing VEGF in a concentration dependent manner（P〈0.05）.We further found that EGCG suppressed tumor growth and the anti-tumor activity of EGCG correlated with a reduction in the expression level of VEGF mRNA and microvessel density in tumor tissue（P〈0.05）. Conclusions EGCG exhibits anti-tumor effect on renal cancer cell by inhibiting proliferation and angiogenesis.