小白蒿总黄酮抗炎作用及其HPLC分离与NMR鉴定

Anti-inflammatory Effects,HPLC Isolation and NRM Identification of the Total Flavonoids from Artemisia frigida

目的:研究小白蒿总黄酮对甲醛致大鼠足肿胀模型的抗炎作用,并明确其主要化学成分。方法:采用甲醛致大鼠足肿胀模型观察小白蒿总黄酮的抗炎作用,然后运用HPLC法对其进行分离并利用NMR法鉴定其分离物。同时,观察小白蒿总黄酮的急性毒性。结果:小白蒿总黄酮在低剂量(50mg/kg)、中剂量(100mg/kg)和高剂量(200mg/kg)下表现出明显的抗炎作用及剂量依赖性关系;根据小白蒿总黄酮的HPLC分离及NMR鉴定结果可知,其中主要含有5个黄酮类化合物,即5,3'-二羟基-3,6,7,4'-四甲氧基黄酮(S1);5,7,3'-三羟基-6,4'-四甲氧基黄酮(S2),;5,3'-二羟基-6,7,4'-三甲氧基黄酮(S3),;5-羟基-3',4'-二甲氧基黄酮-7-O-β-D-葡萄糖醛酸苷(S4);5-羟基-3',4″,5'-三甲氧基黄酮-7-O-β-D-葡萄糖醛酸苷(S5)。小白蒿总黄酮的LD50大于4.06 g/kg。结论:小白蒿总黄酮具有明显的抗炎作用,是小白蒿的主要抗炎有效部位之一。

Objective To study the anti - inflammatory effects of total flavonoids from Artemisia frigida Willd on inflammation in rats, and determine the main chemical components. Methods The anti - inflammatory effects of total fiavonoids from Artemisia frigida Willd on the model of paw edema in rats induced by formaldehyde and then the HPLC and NMR were used to carry on the isolation and identification of lit＇ s chemical components. At the same time, the acute toxicity of total flavonoids from Artemisia frigida Willd was observed. Results At the doses used total flavonoids from Artemisia frigida Willd （50, 100, and 200 mg/kg） showed significant and dose - dependent anti - inflammatory effects. According to the HPLC isolation and NMR identification of the total flavonoids from A. Frigida Willd, there are five major compounds, namely 5, 3＇ - dihydroxy - 3, 6, 7, 4＇ - tetramethoxy flavone （ S1 ） , 5, 7, 3＇ - trihydroxy -6, 4＇ - dimethoxyfiavone （S2） , 5, 3＇ - dihydroxy - 6, 7, 4＇ - trimethoxyflavone （ S3 ） , 5 - hydroxy - 3＇, 4＇ - dime- thoxy - 7 - O - β - D - glucuronide （S4） , 5 - hydroxy - 3＇, 4＇, 5＇ - trimethoxy - 7 - O -β - D - glucuronide （ S5 ）. The LD50 of total flavonoids from Artemisia frigida Willd was more than 4. 06 g/kg. Conclusions The total flavonoids from Artemisia frigida Willd has significant anti - inflammatory effects, which is one of the main active parts of Artemisia frigida Willd.