红景天苷对小鼠宫颈癌移植瘤中Eag1表达的影响和意义

Effect and significance of salidroside on Eag1 expression in mouse cervical cancer xenograft

目的检测红景天苷对小鼠宫颈癌移植瘤中Eag1表达情况的影响及其意义。方法建立小鼠宫颈癌U14细胞皮下移植瘤模型,将40只雌性昆明小鼠分为移植瘤组、顺铂组、红景天苷组和顺铂、红景天苷联合组,每天用游标卡尺测量肿块大小,采用电子天平称量瘤质量并计算抑瘤率,采用免疫组织化学法证实移植瘤为未分化鳞癌,取移植瘤组的瘤旁组织为对照组。采用RT-PCR和Western blot检测各组小鼠宫颈癌移植瘤中Eag1mRNA和蛋白的表达水平。结果皮下注射宫颈癌U14细胞建立昆明小鼠皮下移植瘤模型成瘤率100%。顺铂组、红景天苷组及联合组移植瘤的体积及瘤质量均低于移植瘤组,联合组移植瘤的体积及瘤质量明显低于顺铂组和红景天苷组(P<0.05);移植瘤组、顺铂组、联合组Eag1mRNA和蛋白表达明显高于对照组,顺铂组、红景天苷组、联合组Eag1mRNA和蛋白表达均低于移植瘤组,联合组Eag1表达水平低于顺铂组和红景天苷组,差异具有统计学意义(P<0.05)。结论红景天苷能抑制小鼠宫颈癌移植瘤的生长及Eag1表达,与顺铂联用效果更为显著。

Objective To investigate the effect and significance of salidroside on Eagl expression in mouse cervical cancer xenograft. Methods The mouse subcutaneous xenograft model of cervical cancer U14 cells was established. Forty female Kunming mice were divided into 4 groups according to the random principle, the xenograft group, cisplatinum group, salidroside group and cisplatinum combined salldroside group. Then the mass size was measured by using the vernier caliper everyday. The xenograft tumor weight was measured by using the electronic scale and the tumor inhibiting rate was calculated. The immunohistochemistry method was adopted to verify that the xenograft tumor was undifferentiated squamous eel1 carcinoma. The tumor surrounding tissue in the xenograft group was taken as the control group. RT-PCR and Western blot were adopted to detect the expression levels of Eagl mRNA and protein in mouse cervical cancer xenografts in each group. Results Kunming mice renograft tumor model was established by subcutaneous injection of cervical cancer U14 cells, the tumor formation rate was 100%. The tumor volume and tumor weight in the cisplatinum group,salidroside group and combined group were lower than those in the senograft group,the xenograft tumor volume and tumor weight in the combined xenograft group were significantly lower than those in the cisplatinum group and salidroside group （P〈0.05） ;the expressions of Eagl mRNA and protein in the xenograft group, cisplatinum group and combined group were significantly higher than those in the control group, while which in the cisplatinum group, salidroside group and cisplati- num combined salidroside group were significantly lower than those in the xenograft group, the Eagl expression level in the com- bined group was lower than that in the cisplatinum group and salidroside group, the difference was statistically significant （P〈 0.05）. Conclusion Salidroside can inhibit the growth of cervical cancer xenograft and Eagl expression,and the effect in the com- bined use with cisplatinum is more significant.