凋亡抑制基因Livin在喉鳞状细胞癌中的表达及与预后的关系

The Expression of Livin in Laryngeal Squamous Cell Carcinoma and Its Correlation with Prognosis

目的分析Livin基因在喉鳞状细胞癌组织中的表达情况,并探究其在喉鳞状细胞癌发生、发展及预后中的作用及机制,为喉鳞癌的临床诊断、治疗以及病情预测提供可参考依据。方法选取2013年3月至2016年10月南充市中心医院耳鼻咽喉头颈外科经手术切除的34例喉鳞癌组织、19例癌旁组织和15例正常喉黏膜组织,分别应用SP免疫组化法检查Livin的表达情况,并结合患者的病理结果和随访资料,探究Livin表达与各临床特点以及预后的关系。结果 Livin在喉鳞癌组织中的高表达率为70.59%,在癌旁组织中的高表达率15.79%,在正常喉黏膜组织中无Livin高表达,差异有统计学意义(P<0.05);在喉鳞癌中,Livin高低表达与临床分期、淋巴结是否转移、病理分级以及病程差异有统计学意义(P<0.05),但与患者年龄、性别、肿瘤病灶大小和临床分型差异无统计学意义(P>0.05);经Cox回归性分析发现,Livin阳性表达、病程长、淋巴结转移、病理分级和癌症晚期是影响喉鳞癌患者生存率的独立危险因素。结论 Livin基因的阳性表达在喉鳞癌的发生、发展及预后中发挥着重要作用,可将其作为判断喉鳞癌病情进展及预后的主要指标之一。

Objective To analyze the expression of Livin gene in the tissues of laryngeal squamous carcinoma, investigate the role and mechanism of Livin gene in the occurrence, development and prognosis of iaryngeal squamous cell carcinoma, and provide a reference for the clinical diagnosis and treatment and the disease prediction. Methods The tissues insected from 34 cases of laryngeal carcinoma tissues, 19 cases of paracancerous tissues and 15 cases of normal laryngeal mucosa tissues from March of 2013 to October of 2016 in Nanchong Central Hospital were selected and examined by the SP immunohistochemical method to detect the expression level of Livin. The pathological results and follow-up data were analyzed to investigate the correlation of the Livin expression level with the clinical features and prognosis respectively. Results The high expression rate of Livin in the tissues of laryngeal squamous cell carcinoma and the paracancerous tissues were 70.59 % and 15.79 % respectively and there was no high expression of Livin in the normal laryngeal mucosa tissues. There were significant differences between the three groups （P〈0. 05）. In laryngeal squamous cell carcinoma, the expression level of Livin was correlated with clinical stage, lymph node metastasis, pathological grading and disease course （P〈0.05）, while it was not correlated with age, gender, tumor size and clinical type （P〉0.05）. The results of Cox regression analysis showed that the positive expression of Livin, long disease course, lymph node metastasis, pathological grading and advanced cancer were independent risk factors of the survival rate of the patients with laryngeal squamous cell carcinoma. Conclusion The positive expression of Livin gene plays an important role in the occurrence, development and prognosis of laryngeal squamous cell carcinoma. It can be used as one ol the main indexes to evaluate the progress and prognosis of laryngeal squamous cell carcinoma.