HPA1-6,15基因遗传多态性在血小板输注中的应用研究

Analysis on genetic polymorphism of human platelet antigen HPA1-6,15 systems to platele trtansufsions

目的:调查浙江地区人群HPA系统遗传多态性特点,探讨临床血小板输注中HPA配型输注的应用效果。方法:采用PCR-SSP方法对400例献血员及54例血小板抗体筛检阳性患者进行血小板抗原HPA1-6,15系统基因分型,对54例患者进行分组血小板输注治疗效果分析。结果:血小板抗原HPA1-6,15系统中HPA-3的b基因频率0.51,系统b基因最高,HPA-15的杂合度0.33,系统杂合度最高,其余系统均以aa纯合子基因分型占绝对优势,超过90%。血小板HPA配型输注组CCI、PPR均明显优于ABO随机输注组。结论:在血小板临床输注治疗中应用HPA配型血小板可防止输注无效的发生率,从而有效提高血小板输注疗效和安全性,值得推广和应用。

Objective：To study the genetic polymorphism of human platelet antigens in zhejiang,understand the trtansufsion effect on platele gene matching group in platele trtansufsion.Method：Platelet antigen genotyping was performed by sequence-specific primer polymerase chain reaction（SSP-PCR）for the HPA1-6,15 systems in 400 platele donors and 54 patients of Zhejiang.The effectiveness of differents platelet transfusion for 54 patients of platelet antibody screening positive.Result：Among HPA1-6,15 systems,HPA-3has the highest b gene frequency,its frequency is 0.51,HPA-15 has the highest heterozygosity,HPA-15 ab 0.33,others systems is mainly aa homozygous genotype,more than 90%.Platelet gene matching group has higher CCI and PPR than the group that receives random platelet group.Conclusion：The effectiveness of patients transfusion gene matching platelet prevent from occurrence of platelet transfusion refractoriness and improve the effective rate of transfusion.