摘要
目的探究XCF-43b体外抗血管新生机理。方法鸡胚尿囊膜（CAM）检测化合物抑制血管新生能力．MTY检测其对HUVEC细胞增殖影响，划痕实验和微管形成实验检测化合物对HUVEC迁移和微管形成的影响，Western印迹检测VEGFR2信号通路相关蛋白表达情况。结果XCF-43b能够抑制CAM血管新生，抑制HUVEC细胞增殖的IC50为（28．42±7．23）[zmol／L，对在VEGF刺激下的HUVEC的Ic50值为（9．03±1．28）μmol／L，此外，2．5μmol／L的XCF-43b能够抑制HUVEC细胞的迁移和微管形成；且能够抑制VEGFR2及其下游信号因子的激活。结论XCF-43b通过抑制VEGFR2信号通路来抑制血管新生。
Objective To explore the in vitro anti-angiogenesis mechanism of XCF- 43b. Methods The proliferation of human umbilical vein endothelial cells （HUVEC） was detected by MTF assay and the migration and tube formation of HUVEC were studied by scratches and tube formation. Western blotting was used to detect the expression of VEGFR2 signaling pathway. Results Our results showed that XCF-d3b was able to inhibit angiogenesis by CAM. MTT assay exhibited that XCF-43b could inhibit the proliferation of HUVEC cells. In addition, XCF-43b was found to in- hibit the migration of HUVEC and microtubule formation. Western blot analysis revealed that XCF- 43b was able to inhibit VEGFR2 signaling pathway. Conclusion XCF-43b inhibits angiogenesis by inhibiting VEGFR2 signaling pathway.
出处
《医学分子生物学杂志》
CAS
2017年第3期130-136,共7页
Journal of Medical Molecular Biology
基金
国家自然科学基金（No.U1202221,81560601）,云南省自签科学基金（No.2014FD011）
