丹蒌片对脂肪性肝病合并高脂血症大鼠的保护作用

Effect of Dan Lou Tablet on Blood Lipid Level and Protective Effect in Fatty Liver with Hyperlipidemia in Rats

目的:观察丹蒌片对脂肪性肝病合并高脂血症大鼠的血脂水平的影响及其对大鼠的保护作用。方法:取SPF级大鼠72只,随机分为空白组,模型组,阳性对照组(复方蛋氨酸胆碱片,162 mg·kg^(-1)),丹蒌片大剂量组、中剂量组、小剂量组(900 mg·kg^(-1)、600 mg·kg^(-1)、300 mg·kg^(-1))。空白组给予基础饲料,其余各组低剂量腹腔注射CCl4、灌胃高脂乳剂复制大鼠脂肪性肝病合并高脂血症动物模型。24 d后,尾静脉取血,检测其三酰甘油(triacylglycerol,TG)、总胆固醇(total cholesterol,TC),观察其是否造模成功。造模成功后,各给药组给药28 d,末次给药2 h后,通过HE染色观察肝脏组织病理形态学改变,采用酶联免疫法检测血清TG、TC、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterin,LDL-C)、极低密度脂蛋白(very low density lipoprotein,VLDL)、游离脂肪酸(free fatty acid,FFA)水平,采用酶联免疫法检测肝脏组织匀浆液细胞肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malonaldehyde,MDA)含量水平。结果:与模型组比较,丹蒌片能显著改善脂肪性肝病合并高脂血症引发的肝脏组织及细胞损伤;丹蒌片大剂量组、中剂量组大鼠血清中TG、TC、FFA、LDL-C、VLDL水平显著降低(P<0.05),HDL-C水平显著升高(P<0.05);丹蒌片大剂量组、中剂量组肝脏组织中TNF-α、IL-6、MDA水平显著降低(P<0.05),SOD水平显著升高(P<0.05)。结论:丹蒌片能降低脂肪性肝病合并高脂血症大鼠血脂水平、改善肝组织病理学改变,提示丹蒌片对脂肪性肝病合并高脂血症大鼠具有保护作用,其作用机制可能与丹蒌片能降低血脂水平,降低细胞炎症因子浸润有关。

Objective ：To observe the effect of Dan Lou tablet on lipid levels and protective effects of fatty liver in rats with hyperlip- idemia. Method： 72 rats were rahdomly divided into six groups：sham operation group, myocardial ischemia-reperfusion control group, positive control group（ compound methionine choline tablets, 162 mg·kg-1）, Dan Lou tablet large dose group （900 mg·kg-1 ） ,medium dose group（600 mg·kg-1 ） ,low dose group（300 mg·kg-1 ）. The rats in the other groups were given low dose intraperitoneal injection of CC14, and the rats were injected with hyperlipidemia and hyperlipidemia model. After 24 days, blood was collected from the tail vein and tested for TG and TC. After the model was successfully established, each administration groupwas administered for 28 days, and the last administration of 2 h, the levels of triglyceride （ TG）, total cholesterol （ TC）, high densi- ty lipoprotein （HDL-C） were measured by enzyme-linked immunosorbent assay （ELISA）. The changes of liver histopathological changes were observed by hematoxylin- （LDL-C） ,very low density lipoprotein （VLDL） and free fatty acid （FFA） were measured by enzyme-free method. The levels of tumor necrosis factor-or （TNF-ot）, interleukin （IL-6）, superoxide dismutase （SOD） and ma- londialdehyde （MDA） were measured. Results： Compared with the model control, Dan Lou tablet could significantly improve the liver tissue and cell damage induced by fatty liver and hyperlipidemia. The serum leveis of TG, TC, FFA, LDL-C and VLDL con- tents in the large dose group and medium dose group were decreased（P 〈 O. 05）, and the content of HDL-C was significantly in- creased （P 〈 0.05） and the contents of TNF-a, IL-6 and MDA were significantly decreased （P 〈 0.05）, and the content of SOD increased significantly（P 〈0. 05）. Conclusion： Dan Lou tablet can reduce the level of blood lipids and improve the pathological changes of liver tissue, suggesting that Dan Lou tablet has a protective effect on fatty liver with hyperlipidemia rats,its mechanism may be related to reducing blood lipid levels, decreasing cell infiltration of inflammatory cytokines.