加味补中益气汤对肝郁脾虚型大鼠干预作用的机制

Intervention Effect on Modified Buzhong Yiqi Decoction in Treating Rats of Liver Stagnation and Spleen Deficiency Syndrome

目的:研究加味补中益气汤对肝郁脾虚型大鼠干预作用的机制。方法:取60只Wistar大鼠随机平均分为空白组,模型组,加味补中益气汤高剂量组、中剂量组、低剂量组。除空白组外,各组接受15 d夹尾刺激(每次30 min,4次·d^(-1))和每天大黄灌胃(0.004 5 m L·g^(-1),1次·d^(-1),15 d)。建模后,加味补中益气汤高剂量组、中剂量组、低剂量组分别给予3.8 g·(kg·d)^(-1)、7.6 g·(kg·d)^(-1)和11.4 g·(kg·d)^(-1),空白组、模型组给予等体积的生理盐水溶液,连续10 d,末次给药后1 h,测定血清体积及血清生化指标,分析主要脏器系数。结果:加味补中益气汤高剂量组血清体积比为0.6012,显著高于模型组的血清体积比(0.411 1)(P<0.01);加味补中益气汤高剂量组肝脏系数为6.239 8、脾脏系数为0.4236,显著高于模型组的肝脏系数(4.502 6)和脾脏系数(0.317 1)(P<0.05);加味补中益气汤高剂量组血浆促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)为38.46 ng·L^(-1),显著低于模型组的ACTH(49.27 ng·L^(-1))(P<0.05)。加味补中益气汤中剂量组血清体积比为0.540 3,脾脏系数为0.451 7,均显著高于模型组以上指标(P<0.05);血浆ACTH为45.72 nmol·L^(-1),显著低于模型组ACTH(P<0.01)。加味补中益气汤低剂量组脾脏系数为0.412 8,显著高于模型组脾脏系数(P<0.05)。结论:加味补中益气汤可显著升高大鼠血清体积比、肝脏系数、脾脏系数,降低大鼠ACTH水平,可改善肝郁脾虚型大鼠的脾虚、焦虑、抑郁症状。

Objective ： To investigate the effect of modified Buzhong Yiqi decoction in treating liver stagnation and spleen deficiency rats. Methods：60 Wistar rats were randomly divided into 5 groups, the blank group, model group, modified Buzhong Yiqi decoction high dose group, middle dose group,low dose group. In addition to the normal group, each group received stimulation of pinching tail（30 min per time,4 times a day） and was administered rhubarb （0. 004 5 mL · g-1,1 time a day） via gavage daily for 15 days. After molding, modified Buzhong Yiqi decoction groups were treated with the dose of 3.8 g·（kg·d）-1 - 1,7.6 g·（kg·d）-1 -1, and 11.4 g · （kg · d） -1, respectively. The blank group and model group were treated with physiological saline for 10 days. The last 1 h after treatment, serum volume and serum biochemical index were measured and main organ coefficients were analyzed. Results ： In terms of the high dose group of modified Buzhong Yiqi decoction, the serum volume ratio was 0. 601 2, markedly higher than that of the model group （0. 411 1 ） （P 〈0.01 ） ;the liver coefficient was 6. 239 8 ,the spleen coefficient was O. 423 6, bothhigher than those of the model group （4.502 6,0.317 1 ） （P 〈 0.05 ） ;the adrenocorticotropic hormone （ACTH） in plasma was 38.46 ng · L-· ,obviously lower than that of the model group （49.27 ng · L-1 ） （P 〈0.05）. The serum volume ratio of the mid- die dose group of modified Buzhong Yiqi decoction was 0. 540 3, the spleen coefficient was 0.451 7, both obviously higher than those of the model group （P 〈 0.05 ） ; the ACTH in plasma of the middle dose group was 45.72 nmol · L- 1, obviously lower than that of the model group （P 〈 0.01 ）. The spleen coefficient of the low dose group was 0.412 8, obviously higher than that of the model group （P 〈 0.05 ）. Conclusion ： Modified Buzhong Yiqi decoction can increase the serum volume ratio of rats, liver index, spleen index and decrease the level of ACTH in rats,improve spleen defieiency, anxiety and depressive symptoms of liver depres- sion and spleen deficiency rats.