泽泻、郁金、熟地黄合煎对熟地黄中毛蕊花糖苷煎出量的影响

Effects of Combined Decoctions of Alismatis Rhizoma and Curcumae Radix and Rehmanniae Radix Praeparata on the Decoction Amount of Acteoside in Rehmanniae Radix Praeparata

目的:研究泽泻、郁金、熟地黄合煎对熟地黄中毛蕊花糖苷煎出量的影响,为三药合用的药效物质研究提供参考。方法:取药材分为单煎-1(熟地黄20 g)、单煎-2(泽泻20 g)、单煎-3(郁金20 g)、合煎-1(熟地黄、泽泻各20 g)、合煎-2(熟地黄、郁金各20g)、合煎-3(泽泻、郁金各20 g)、合煎-4(熟地黄、泽泻、郁金各20 g)组,分别制备水煎液干浸膏,回流法提取后采用高效液相色谱法检测样品液中毛蕊花糖苷的含量并计算其煎出量。结果:单煎-1、合煎-1、合煎-2、合煎-4干浸膏中毛蕊花糖苷煎出量分别约为0.035 4、0.022 3、0.022 8、0.011 0 mg/g,后3组与单煎-1组比较差异具有统计学意义(P<0.01),以三药合煎组煎出量最低;阴性对照组(单煎-2、单煎-3及合煎-3)中未检测到毛蕊花糖苷。结论:熟地黄与泽泻、郁金三药合煎时毛蕊花糖苷煎出量降低,推测毛蕊花糖苷可能不是三药合煎液中发挥药效作用的主要成分。

OBJECTIVE： To study the effects of combined decoctions of alismatis rhizoma and curcumae radix and rehmanniae radix praeparata on the decoction amount of acteoside in rehmanniae radix praeparata, and provide reference for studying the effec- tive ingredients of three-drug effect. METHODS： Single decoction-1 （rehmanniae radix praeparata 20 g）, single decoction-2 （alis- matis rhizoma 20 g）, single decoction-3 （curcumae radix 20 g）, combined decoction-1 （rehmanniae radix praeparata 20 g, alisma- tis rhizoma 20 g）, combined decoction-2 （rehmanniae radix praeparata 20 g, curcumae radix 20 g）, combined decoction-3 （alisma- tis rhizoma 20 g, curcumae radix 20 g） and combined decoction-4 （rehmarmiae radix praeparata 20 g, alismatis rhizoma 20 g, cur- cumae radix 20 g） were respectively taken to prepare dry extracts after extracting by refluxing, HPLC was used to detect the acteo- side content and calculate its decoction amount in sample. RESULTS： The decoction amounts of acteoside in single decoction-l, combined decoction-i, combined decoction-2 and combined decoction-4 dry extracts were 0.035 4, 0.022 3, 0.022 8, 0.011 0 mg/ g, respectively. Compared with single decoction-1 group, the last 3 groups had statistical significances （P〈0.01）; combined decoc- tion-4 showed lowest decoction amount in three-drug combined decoction group. Acteoside was not detected in the negative control groups （single decoction-2, single decoction-3 and combined decoction-3）. CONCLUSIONS： The decoction amount of acteoside is reduced when alismatis rhizoma, curcumae radix and rehmanniae radix praeparata were decocted together, indicating that it may not be the main ingredient of playing effects in three-drug combined decoction liquid.