葛根素配伍丹酚酸B注射使用对大鼠心肌缺血再灌注损伤的保护作用

Protective Effect of the Injection of Puerarin Combined with Salvianolic Acid B on Rats with Myocardial Ischemia Reperfusion Injury

目的:研究葛根素配伍丹酚酸B注射使用对大鼠心肌缺血再灌注损伤(MIRI)的保护作用。方法:将62只大鼠随机分为假手术组、模型组、葛根素组(20 mg/kg)和葛根素(20 mg/kg)-丹酚酸B不同配比组(质量比分别为1∶0.5、1∶1、1∶2),每组10只。除假手术组外,其余各组大鼠复制MIRI模型。再灌注180 min后,检测大鼠血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平及心肌梗死面积百分比。结果:与假手术组比较,模型组大鼠血清中CK、LDH、MDA水平明显升高(P<0.01),SOD水平明显降低(P<0.01);心肌梗死面积百分比明显升高(P<0.01)。与模型组比较,各给药组大鼠血清中CK、LDH、MDA水平明显降低(P<0.05或P<0.01),SOD水平明显升高(P<0.05或P<0.01),其中葛根素-丹酚酸B(1∶1)组指标变化最为明显;心肌梗死面积百分比明显降低(P<0.01),其中葛根素-丹酚酸B(1∶1)组和(1∶2)组小鼠的心肌梗死面积小于葛根素注射剂组(P<0.01)。结论:葛根素注射剂配伍丹酚酸B后较单用葛根素注射剂能更有效地抑制MIRI后心肌细胞损伤、减小心肌梗死面积,且以质量比为1∶1时效果最优。

OBJECTIVE： To study the protective effect of the injection of puerarin combined with salvianoli acid B （Sal B） on rats with myocardial ischemia reperfusion injury （MIRI）. METHODS： 62 rats were randomly divided into sham operation group, model group, puerarin group （20 mg/kg） and puerarin （20 mg/kg）-Sal B group （mass ratio of 1：0.5, 1：1, 1：2, respectively）, 10 in each group. Except for sham operation group, rats in other groups were reduced for MIRI model. After 180 rain of reperfusion, kinase （CK）, lactate dehydrogenase （LDH）, superoxide dismutase （SOD）, malondialdehyde （MDA） in serum and percentage of myocardial infarction size of rats were detected. RESULTS ： Compared with sham operation group, CK, LDH, MDA levels in se- rum of rats in model group were obviously increased （P〈0.01）, SOD level was obviously decreased （P〈0.01） ; and percentage of myocardial infarction size was obviously increased （P〈0.01）. Compared with model group, CK, LDH, MDA levels in serum of rats in each administration group were decreased （P〈0.05 or P〈0.01）, SOD levels were obviously increased （P〈0.05 or P〈 0.01 ）, and indexes changed the most obviously in puerarin-Sal B group （1 ： 1） ; percentage of myocardial infarction size was obvi- ously decreased （P〈 0.01 ）, and the percentage of myocardial infarction sizes in puerarin-Sal B group （ 1 ： 1 ） and group （ 1 ： 2） were less than Puerarin injection group （P〈0.01）. CONCLUSIONS： Compared with Puerarin injection alone, puerarin combined with Sal B by injection can more effectively inhibit the cardiomyocyte injury and decrease myocardial infarction size after MIRI, with best efficacy when quality ratio is 1 ： 1.