胃得安片对应激性胃损伤模型大鼠胃功能及胃组织中EGF、EGFR表达的影响

Effect of Weide'an Tablet on Gastric Function and the Expressions of EGF and EGFR in Gastric Tissue of Model Rats with Stress-induced Gastric Injury

目的:研究胃得安片对应激性胃损伤模型大鼠胃功能及胃组织中表皮生长因子(EGF)及其受体(EGFR)表达的影响,探讨其治疗胃溃疡的作用机制。方法:将大鼠随机分为正常组(生理盐水)、模型组(生理盐水)、阳性组(盐酸雷尼替丁,0.015 mg/kg)和胃得安片高、中、低剂量组(1.7、0.87、0.43 g/kg),每组10只。除正常组外,其余各组大鼠均采用空腹冷水游泳法复制胃溃疡模型。成模后,各组大鼠每天ig给药1次,连续2周。记录大鼠于给药第0、7、14天时的体质量;末次给药12 h后,检测大鼠的胃液分泌量、胃液pH值、胃溃疡面积及胃黏膜损伤指数,并检测胃组织中EGF、EGFR的表达。结果:与正常组比较,模型组大鼠给药第7、14天时的体质量及胃液pH值降低,胃液分泌量和胃溃疡面积增加,胃黏膜损伤指数升高,胃组织中EGF、EGFR表达增强,差异均有统计学意义(P<0.05或P<0.01)。与模型组比较,除胃得安片中剂量组大鼠给药第7天时的体质量、胃液分泌量、胃液pH值、胃溃疡面积以及胃得安片低剂量组大鼠给药第7、14天时的体质量、胃液分泌量、胃液pH值、胃溃疡面积改善和胃组织中EGF表达增强不显著外,其余各给药组大鼠上述指标均显著改善,且胃组织中EGF、EGFR表达持续增强(P<0.05或P<0.01);胃得安片的作用呈现一定的量效关系。结论:胃得安片能够显著改善应激性胃损伤模型大鼠的胃功能;其机制可能与增强胃组织中EGF、EGFR表达,从而促进溃疡愈合有关。

OBJECTIVE ： To study the effects Weide＇ an tablet on gastric function and the expressions of epidermal growth fac- tor （EGF） and its receptor （EGFR） in gastric tissue of model rats with stress-induced gastric injury, and investigate its mechanism in the treatment of gastric ulcer. METHODS： Rats were randomly divided into normal group （normal saline）, model group （nor- mal saline） , positive group （ranitidine hydrochloride, 0.015 mg/kg） and Weide＇ an tablet high-dose, medium-dose, low-dose groups （1.7, 0.87, 0.43 g/kg）, 10 in each group. Except for normal group, rats in other groups were given fasting swimming in cold water to induce gastric ulcer model. After modeling, all rats were intragastrically administrated once a day, for 2 weeks. The body mass of rats in 0, 7, 14 d of administration was recorded. After 12 h of last administration, gastric juice secretion, gastric juice pH, gastric ulcer area and gastric mucosal damage index of rats were detected, and the expressions of EGF and EGFR in gas- tric tissue were detected. RESULTS： Compared with normal group, body mass and gastric juice pH in 7, 14 d in model group were declined; gastric juice secretion and gastric ulcer area were increased, gastric mucosal damage index was increased; and the expressions of EGF and EGFR in gastric tissue were enhanced, with statistical significances （P〈0.05 or P〈0.01）. Compared with model group, except that the body mass, gastric juice secretion, gastric juice pH and gastric ulcer area in Weide＇ an medium-dose group in 7 d, and body mass, gastric juice secretion, gastric juice pH, gastric ulcer area and the expression of EGF in gastric tis- sue in Weide＇an low-dose group in 7, 14 d were not significantly improved, above indexes were significantly improved in other ad- ministration groups （P〈0.05 or P〈0.01）; and the effect of Weide＇ an tablet showed a certain dose-effect relationship. CONCLU- SIONS： Weide＇ an tablet can significantly improve the gastric fimction of model rats with stress-induced gastric injury; and the mechanism may be related with enhancing the expressions of EGF and EGFR and promoting ulcer healing.