洋金花茎化学成分的分离鉴定

Isolation and Identification of Chemical Constituents from Stems of Datura metel

目的:研究洋金花Datura metel茎的化学成分。方法:采用硅胶,ODS柱色谱及HPLC等色谱分离方法进行分离纯化,利用NMR,HR-ESI-MS等波谱学方法结合参考文献鉴定化合物的结构。结果:从洋金花茎70%乙醇提取物的乙酸乙酯萃取部位中分离得到14个化合物,分别鉴定为N-[2-(3,4-dihydroxyphenyl-2-hydroxyethyl)]-3-(4-methoxyphenyl)prop-2-enamide(1),3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxyphenyl)-2-methoxyethyl]acrylamide(2),N-反式-对-香豆酰基章鱼胺(3),N-顺式阿魏酰基酪胺(4),N-trans-feruloyl-3',4'-dihydroxyphenylethylamine(5),N-反式阿魏酰基酪胺(6),N-反式-对-香豆酰基酪胺(7),草夹竹桃苷(8),daturataturin A(9),baimantuoluoside H(10),daturametelin A(11),柑橘苷A(12),9,12,13-三羟基-10,15-十八碳二烯酸甲酯(13),(9E)-8,11,12-三羟基-十八碳烯酸甲酯(14)。结论:化合物1,8为首次从茄科植物中分离得到,化合物2~5为首次从曼陀罗属植物中分离得到。

Objective： To study the chemical constituents from the stems of Datura metel. Method： The compounds were isolated and purified by silica gel and ODS chromatogram columns together with semi-preparative HPLC. The structures of the isolated compounds were identified by ^1H-NMR and ^13C-NMR as well as HR-ESI-MS data. Result： Fourteen compounds were isolated from the ethyl acetate fraction of the 70% ethanol extract from the stems of D. metel, which were identified as N-[ 2-（ 3, 4-dihydroxyphenyl-2-hydroxyethyl ）]-3-（ 4- methoxyphenyl） prop-2-enamide （ 1 ）, 3- （ 4-hydroxy-3-methoxyphenyl ） -N- [ 2- （ 4-hydro-xyphenyl ） -2- methoxyethyl] acrylamide （2）, N-trans-p-coumaroyloctopamine （3）, N-cis-feruloyhyramine （4）, N-trans- feruloyl-3＇, 4＇-dihy-droxyphenylethylamine （5）, N-trans-feruloyl tyramine （6）, trans-N-p-coumaroyhyramine （7）, androsin （8）, daturataturin A （9）, baimantuoluoside H （10）, daturametelin A （11）, citroside A （12）, methyl-9, 12, 13-trihydroxyoctadeca-10, 15-dienoate （13） and （9E） -8, 11, 12-trihydroxyoctadecenoic acid methyl ester （14）. Conclusion： Compounds 1 and 8 were isolated from the plants of Solanaceae for the first time and compounds 2-5 were isolated from Datura genus for the first time.