泰索帝对Lewis肺癌放疗增敏作用的实验研究

THE RADIOSENSITIZATION OF DOCETAXEL ON LEWIS LUNG CANCER IN VIVO

研究了泰索帝 (docetaxel)对荷Lewis肺癌C5 7BL小鼠的放疗增敏作用。给荷Lewis肺癌C5 7BL小鼠腹腔注射不同剂量的泰索帝 12h或 4 8h后进行 10Gy局部照射。结果表明 ,经过泰索帝放疗增敏治疗后肿瘤生长相对速率明显减慢 ,4个放疗增敏组q值在 1.2 1— 1.38之间。研究还显示泰索帝的放疗增敏作用机制是通过将大量增殖期细胞阻滞在G2 和M期以及诱导细胞凋亡。本实验提示泰索帝作为放疗增敏剂应用于肺癌的治疗 ,既发挥了化疗作用 ,又具有放疗增敏作用 。

In this paper the radiosensitization of docetaxel on Lewis lung cancer was investigated in vivo. C57BL mice bearing Lewis lung cancer were treated with 207mg/m 2 docetaxel to determine the ability of the drug to induce mitotic arrest and apoptosis in tumor cells. The mice bearing Lewis lung cancer in the right hind leg were locally irradiated with single dose of 10Gy 137 Cs γ rays using small animal irradiator. The results showed that the percentage of arrested cells increased rapidly with the time, achieving its peak of 31.9% 12h after docetaxel administration. Docetaxel also induced apoptosis within 48h. Treated by different doses of docetaxel 12 or 48h prior to irradiation, the tumors grew slowly, the growth inhibition rates of the tumors increased, and the relative growth rates decreased significantly. The q value in every radiosensitizing group ranged from 1.21 to 1.38. These results indicated that docetaxel exerted its radiosensitizing potential by the mechanism of arresting mitosis and inducing apoptosis. This study suggested that docetaxel had a high potential as a successful radiosensitizer in the clinic treatment of lung cancer.