红松松针精油抗氧化和抑菌活性研究

Antioxidant and antibacterial activity of essential oil from Pinus koraiensis needles

本文以红松松针精油为研究对象,主要研究了红松松针精油的抗氧化和抑菌活性。通过对DPPH自由基、ABTS自由基、超氧阴离子(O_2^(-·))自由基的清除活性以及总还原力探讨红松松针精油的抗氧化活性;以大肠杆菌、枯草芽胞杆菌和金黄色葡萄球菌为供试菌种,测定抑菌圈和最低抑菌浓度来研究红松松针精油的抑菌活性。实验结果显示:该精油对DPPH自由基、ABTS自由基、超氧阴离子(O_2^(-·))自由基的半数清除率IC_(50)值分别为4.24、4.46、3.26 mg/m L;精油对大肠杆菌、枯草芽胞杆菌和金黄色葡萄球菌的抑菌圈分别为(15.2±0.12)mm、(13.3±0.15)mm、(9.5±0.21)mm,最低抑菌质量浓度分别为1.26、2.50、5.00μL/m L。实验结果证明,红松松针精油作为天然抗氧化、抑菌剂具有巨大的开发潜质。

Taking essential oil from Pinus koraiensis needles as the research object, its antioxidant and antimicrobial activities were evaluated. The antioxidant activity of the essential oil was explored with DPPH free radicals,ABTS free radicals,super-oxide anion free radical（ O_2^（-·）） and total reducing power as indicators. Antibacterial activity of the essential oil was studied with Escherichia coli,Bacillus subtilis and Staphylococcus aureus as the test strain against typical food-borne microorganisms,with minimum inhibitory concentration. Pine needle essential oil showed remarkable antioxidant activity in scavenging DPPH free radical,ABTS free radicals,super-oxide anion free radical（ O_2^（-·））,and in reducing power assays. Essential oils of half of the clearance IC_（50） value concentration were 4. 24,4. 46,3. 26 mg/mL.The diameters of Escherichia coli,Bacillus subtilis and Staphylococcus aureus inhibition zone were（ 15. 20 ±0. 12） mm,（ 13. 30 ± 0. 15） mm,and（ 9. 50 ± 0. 21） mm,and the minimum inhibitory concentration（ MIC） was 1. 26,2. 50,and 5. 00 μL/mL. These results suggest that the essential oil from pine needles has potential to be used as a natural antioxidant and antimicrobial agent in food processing.