一起腺病毒引起的聚集性呼吸道感染的实验室调查

Laboratory investigation of a clustered respiratory tract infection with human adenovirus

目的对一起幼儿园聚集性发热事件开展病原学调查,为疫情处置提供依据。方法采集部分患者呼吸道样品,应用real-time RT-PCR法筛查,腺病毒核酸阳性样品接种HEp-2细胞系,PCR扩增部分腺病毒Hexon高变区基因片段并测序,确定病毒型别并进行变异分析。结果从10份咽拭子标本中检测到7份腺病毒核酸阳性。经分离培养得到7株人腺病毒毒株。系统进化分析确定均为人腺病毒7型。推导的氨基酸序列分析显示,与近年来国内外流行株相比,分离株的Hexon蛋白高变区无氨基酸位点突变。结论结合病例临床表现以及流行病学调查结果,此事件证实系人腺病毒7型引起的急性呼吸道感染聚集性事件。

In order to investigate the pathogens associated with a clustered event of fever occurred in a kindergarten in Fuzhou,Fujian Province,samples were collected from pediatric cases in the kindergarten and screened for various possible viral agents by real-time PCR.Of 10 respiratory specimens,7 were positive of human adenovirus（HAdV）.The positive samples were inoculated into HEp-2 cell-lines for viral isolation.The PCR products of the hypervariable regions of Hexongene were sequenced,followed by BLAST searches for viral type identification.In comparison with the strains prevalent in domestic or abroad in recent years,the deduced amino acid sequences showed no amino acid mutation in the hypervariable regions of Hexon.Combined with clinical manifestation and field epidemiological data,human adenovirus type 7 could be confirmed as the pathogen linked to the clustered event.