摘要
受激发射损耗(STED)超高分辨率成像技术是一种突破光学衍射极限的显微镜技术,可以达到纳米级分辨率,适合于对200 nm以内的细胞细微结构进行观察和分析。STED技术的优势包括超高分辨率、快速扫描、多色观察、活细胞监测、厚组织标本3D重建、共定位分析等,是现代医学和细胞生物学研究的理想工具。目前STED技术成功实现了对于荧光纳米颗粒的成像,更多的亚细胞结构细节以及活细胞器的生理活动可以被STED技术解析出来,得到了越来越广泛的应用。文章主要介绍了STED超高分辨率成像技术的主要原理及其在医学细胞生物学研究领域的主要应用。
Stimulated emission depletion based super-resolution imaging technology is a microscopic technique, which breaks the optical diffraction limit with a resolution of nanoscale. It is used for the observation andanalysis of microscopic structures less than 200 nm in cells. The advantages of STED technique include the super-resolution, rapid scanning, multi-color observation, live cells monitoring, 3D reconstruction of thick tissues, and co-localization analysis, which is growing up to be an ideal tool in the field of medical and cell biological research. STED has been successfully applied to take pictures of in fluorescentantiparticle, more and more subcellular details and physiological activities of living organelles can be resolved by STED technology, which is being widely used. This is a brief overviews for the working principle of STED based super-resolution imaging technology and its application in the field of medical cell biology.
出处
《中国继续医学教育》
2017年第18期202-204,共3页
China Continuing Medical Education
基金
2015年辽宁省自然科学基金项目(2015020739)
关键词
STED技术
工作原理
医学细胞生物学
应用
stimulated emission depletion super-resolution imaging technology
working principle
medical cell biological
application