麻黄细辛附子汤对变应性鼻炎大鼠INF-γ与IL-13含量的影响

The Influence of Ephedra and Aconite and Asarum Decoction on Contents of INF-γ and IL-13 of Rats with Allergic Rhinitis

目的:研究麻黄细辛附子汤对变应性鼻炎(allergic rhinitis,AR)大鼠INF-γ与IL-13的影响,揭示麻黄细辛附子汤治疗AR的作用机制。方法:将40只wistar大鼠按体质量采用随机区组法分成5组,即正常对照组、模型对照组、扑尔敏治疗组、麻黄细辛附子汤低剂量组、麻黄细辛附子汤高剂量组,每组8只,造模分为2个阶段,即基础致敏与鼻内激发。基础致敏:实验组使用5%OVA混悬液致敏,腹腔注射,日1次,共7次;对照组以等量生理盐水替代5%OVA混悬液,方法和步骤同实验组。鼻内激发:实验组从第15天起,以5%卵清蛋白(ovalbumin,OVA)混悬液100μL双侧滴鼻,每侧50μL,日1次,共7次;对照组以等量生理盐水替代5%OVA混悬液,方法和步骤同实验组。麻黄细辛附子汤低剂量组:使用麻黄细辛附子汤2 m L·次^(-1)(每毫升含生药0.2 g),1日2次灌胃,共21 d。麻黄细辛附子汤高剂量组:以麻黄细辛附子汤2 m L·次^(-1)(每毫升含生药0.4 g),一日2次灌胃,共21 d。模型组及正常对照组分别使用生理盐水2 m L·次^(-1),日2次灌胃,共21 d。扑尔敏治疗组:0.01%扑尔敏溶液2 m L·次^(-1),日2次灌胃,共21 d。检测麻黄细辛附子汤治疗后血浆INF-γ与IL-13的含量。结果:模型组INF-γ含量(17.4±2.7)μg·L-1显著降低,IL-13(50.3±2.6)μg·L-1显著升高,治疗后麻黄细辛附子汤低剂量组和麻黄细辛附子汤高剂量组INF-γ含量(22.6±3.0;20.4±2.5)μg·L-1含量升高,IL-13(35.0±3.0;34.2±3.3)μg·L-1含量降低。模型组与正常对照组比较有统计学意义(P<0.01)。麻黄细辛附子汤治疗组、扑尔敏治疗组与模型组比较有统计学意义(P<0.01)。AR大鼠INF-γ含量较低,而IL-13含量较高,与正常对照组比较有统计学意义(P<0.01)。结论:麻黄细辛附子汤可以调节免疫失衡功能,通过使INF-γ含量升高,使IL-13含量降低纠正体内Thl和Th2表达失衡而发挥疗效。

Objective： To study the influence of Ephedra and Aconite and Asarum Decoction on contents of INF-γ and IL-13 of rats with allergic rhinitis, and reveal its mechanism in the treatment of allergic rhinitis （AR） . Methods： 40 wistar rats were divided into 5 groups according to weight by using random blocking method： normal control group, model control group, chlorpheniramine group, low-dose group of Ephedra and Aconite and Asarum Decoction, high-dose group of Ephedra and Aconite and Asarum Decoction, with 8 rats in each group. The model-establishing was divided into 2 stages, namely the basic sensitization and intranasal provocation. The basic sensitization： The experimental group was sensitized by 5% OVA suspension, injected intraperitoneally 1 time a day, for 7 times together, while the control group used the same amount of normal saline to replace 5% OVA suspension. The method and procedure were the same as the experimental group. The intranasal provocation： From the fifteenth day, the experimental group was given 100μl of 5% OVA suspension for dripping bilateral nose, each side 50μl, 1 times a day, for 7 times together, while the control group used the same amount of normal saline to replace 5% OVA suspension. The method and procedure were the same as the experimental group. Low -dose group of Ephedra and Aconite and Asarum Decoction used 2 ml of the decoction each time （each milliliter containing crude drug 0. 2 g） for intragastric administration, twice a day, for 21 days together. High -dose group of Ephedra and Aconite and Asarum Decoction used 2 ml of the decoction each time （ each milliliter containing crude drug 0.4 g） for intragastric administration, twice a day, for 21 days together. The model group and normal control group were treated with physiological saline 2 mL each time, twice a day for intragastric administration, for 2 days together. Chlorpheniramine group was treated with 2 mL chlorphenirmnine solution with O. 01% concentration each time, twice a day for intragastric administration, for 2 days together. Then the contents of INF- gamma and IL-13 in plasma were detected after being treated with Ephedra and Aeonite and Asarum Decoction. Results： The model group＇ s INF-gamma content （ 17. 4 ±2.7） ng·L^-1 decreased significantly, IL-13 （50.3± 2.6） ng ·L^-1 increased significantly. After the treatment, the contents of INF-gamma in low-dose group of Ephedra and Aconite and Asarum Decoction, and high-dose group of Ephedra and Aconite and Asarum Decoction： （22：6± 3.0;20.4 ± 2.5） ng·L^-1 increased, while the contents of IL-13 （ 35 ± 3 ; 34.2 ±3.3 ） ng · L^-1 decrased. Compared with the normal Control group, the model group was statistically significant （P 〈0.01 ）, Ephedra and Aco- nite and Asarum Decoction treatment groups, chlorpheniramine group, and model control group were statistically significant （ P 〈 0. 01 ）. The content of INF- gamma in AR rats was lower, but the content of IL-13 was higher, which was statistically significant compared with the normal control group （P 〈0.01 ）. Conclusion： Ephedra and Aconite and Asarum Decoction can modulate the imbalance of immune function, correct the imbalance of Thl and Th2 expression in the body by increasing the content of INF- gamma, and decreasing the content of IL-13.