百合知母汤对支气管哮喘大鼠气道炎症的影响

Effect of Baihe Zhimu Tang(百合知母汤) on Airway Inflammation in Bronchial Asthma Rats

目的探讨百合知母汤对支气管哮喘大鼠气道炎症的干预作用。方法 40只Wistar大鼠随机分为空白对照组、哮喘模型组、百合知母汤组、地塞米松组,每组10只。除空白对照组外其余各组大鼠采用10%卵白蛋白(OVA)腹腔注射以及2%OVA超声雾化吸入建立支气管哮喘大鼠模型。每次雾化之前30 min,百合知母汤组给予百合知母汤3.68 g/(kg·d)灌胃,地塞米松组给予地塞米松注射液1.2 mg/(kg·d)灌胃,空白对照组和哮喘模型组则给予同体积生理盐水灌胃,连续干预14天。HE染色观察大鼠肺组织病理情况,检测血浆白细胞介素2(IL-2)、白细胞介素4(IL-4)含量,分离外周血中淋巴细胞观察细胞周期情况及钙调神经磷酸酶(CaN)活性。结果哮喘模型组大鼠光镜下支气管管壁内可见大量炎性细胞浸润,血浆IL-4含量较空白对照组增加,IL-2含量降低,G_0/G_1期淋巴细胞所占比例降低,S期与G_2/M比例增加,淋巴细胞CaN活性显著升高(P<0.05)。百合知母汤组大鼠肺组织光镜下仅见少量炎性细胞浸润,较哮喘模型组血浆中IL-4含量降低,IL-2含量升高,G_0/G_1期淋巴细胞所占比例增加,S期与G_2/M比例显著减少,淋巴细胞CaN活性显著降低(P<0.05)。结论百合知母汤可能通过降低支气管哮喘大鼠外周血淋巴细胞CaN活性,从而抑制淋巴细胞增殖,降低IL-4含量,减轻气道炎症。

Objective To explore the effect of Baihe Zhimu Tang（ 百合知母汤,BZT） on the airway inflammation in bronchial asthma rats. Methods Forty Wistar rats were randomized into control group,asthma model group,BZT group and dexamethasone（ DX） group with 10 rats in each group. The bronchial asthma rat model was built by intraperitoneal injection of 10% ovalbumin（ OVA） and ultrasonic atomizing inhalation of 2% OVA except for the control group. Thirty minutes before every ultrasonic atomizing inhalation,the BZT group was given BZT 3. 68 g/（ kg·d） by gastric perfusion; the DX group was administered DX injection 1. 2 mg/（ kg·d） by gastric perfusion; as for the control group and asthma model group,they were given normal saline of the same volume; all groups were intervened for 14 days. After that,HE staining was used to observe the pathological changes of lung tissue,the levels of interleukin-2（ IL-2） and interleukin-4（ IL-4） were measured,and the cell cycle and calcineurin（ CaN） activity were observed in separated peripheral blood lymphocytes. Results For the asthma model group,there was a lot of inflammatory cell infiltration in the bronchial wall under light microscope,the content of IL-4 in the plasma was higher than that in the control group,while the content of IL-2 decreased,the proportion of G_0/G_1 phase lymphocytes decreased,the proportion of S phase and G_2/M increased,and the CaN activity of lymphocytes increased significantly（ P〈0. 05）. For the BZT group,there was only a small amount of inflammatory cell infiltration in the lung tissue under light microscope,the IL-4 level in the plasma was lower than that in the asthma model group,while the IL-2 content increased,the proportion of G_0/G_1 phase lymphocytes increased,the proportion of S phase and G_2/M decreased significantly,and the CaN activity of lymphocytes decreased significantly（ P〈0. 05）. Conclusion BZT could reduce lymphocyte proliferation,decrease IL-4 level and reduce airway inflammation by reducing CaN activity in peripheral blood lymphocytes of bronchial asthma rats.