氯胺酮对产前束缚应激子代雄性大鼠抑郁样行为的影响

Effects of ketamine on depression-like behaviors of male offspring rat exposed to prenatal restraint stress

目的探讨氯胺酮对产前束缚应激子代大鼠不同发育阶段的抑郁样行为的影响。方法随机选择怀孕的SD母鼠分成对照组（n=6）和产前束缚应激组（n=8）。产前束缚应激组的母鼠每天接受3次（45min／次）束缚应激。检测两组母鼠的后代在幼年期、青春期、成年早期的焦虑和抑郁样行为，并观察氯胺酮对不同发育阶段的产前应激子代大鼠的抗抑郁效果。结果在旷场实验中，不同发育阶段的产前束缚应激子代大鼠中心区域活动时间[幼年期（2．50＋0．43）S、青春期（9．17±1．05）s、成年早期（8．33±0．92）s]均明显低于对照组[（8．33±1．05）S、（19．17±1．06）s、（18．83±1．30）S；均P〈0．05]。在强迫游泳测试中，不同发育阶段的产前束缚应激子代大鼠不动时间[幼年期（192．50±10．82）S、青春期（182．75±10．12）S、成年早期（199．88±9．20）S]均明显高于对照组[（76．00±19．00）S、（96．30±12．91）S、（108．30±10．98）s；均P〈0．05]。氯胺酮能快速且强烈地减少青春期、成年早期产前应激束缚子代大鼠的不动时间（P〈0．01），而对幼年期产前束缚应激子代大鼠不动时间的作用较弱（P〈0．05）。结论产前束缚应激能使子鼠出现持续性的焦虑和抑郁样行为，氯胺酮对青春期和成年早期的产前束缚应激子代大鼠能起到良好的抗抑郁效果。

Objective To investigate the effect of ketamine on depression-like behaviors at different developmental stages of offspring rat exposed to prenatal restraint stress （PRS）. Methods Pregnant SD rats were randomly divided into control group （n= 6） and PRS group （n： 8）. The dams of PRS group received three times（ 45 minutes/time）restraint stress every day. The anxiety-like and depression-like behaviors of the offsprings of the two groups were tested in the stage of juvenile, adolescence and early adulthood. Then the antidepressant effect of ketamine on prenatal stress rats at different developmental stages was observed. Re- suits In the open-field test, the time in the central area of the offspring rats in PRS group at different devel- opmental stages （ juvenile（ 2.50±0.43 ） s, adolescence （ 9.17±1.05 ） s, early adulthood （8.33±0.92） s ） were significantly lower than those of the control group（ （ 8.33± 1.05 ） s, （ 19.17± 1.06） s, （ 18.83± 1.30 ） s, all P〈 0.05）. In the forced swimming test,the immobility time in the offspring rats of PRS group at the different devel- opmental stages （juvenile （ 192.50± 10.82） s, adolescence （ 182.75± 10.12） s, early adulthood （199.88±9.20） s ） were significantly higher than those of control group （ （ 76.00± 19.00） s, （ 96.30±12.91 ） s, （ 108.30± 10.98） s, all P〈0.05 ）. Ketamine could quickly and strongly reduce the immobility time of the offsprings exposed to PRS in the stage of adolescence and early adulthood （P〈0.01） ,but the effect was weaker in the juvenile offsprings （P〈0,05）. Conclu- sion PRS leads to persistent anxiety-like and depression-like behavior in offsprings and ketamine exerts a good antidepressant effect on the offspring rats in the stage of adolescence and early adulthood.