陕西省定边县鼠疫疫区啮齿动物巴尔通体感染情况调查

Investigation of the Bartonella infection in wild rodent populations in Dingbian plague areas of Shaanxi

目的了解陕西省定边县鼠疫疫区啮齿动物巴尔通体感染情况及携带种类,为巴尔通体感染的预防控制提供科学依据。方法 2015年在定边县鼠疫疫区的2个乡镇捕获啮齿动物,无菌取鼠脾,分离培养巴尔通体,疑似菌株提取DNA,PCR扩增glt A基因,测序并根据序列应用Mega 6.06软件进行系统发育分析,确定巴尔通体属种。结果共捕获3种223只啮齿动物,分离巴尔通体78株,感染率为34.98%,其中主要啮齿动物长爪沙鼠的感染率为34.72%;序列分析表明定边县鼠疫疫区共检出4个巴尔通体种群:伊丽莎白巴尔通体(B.elizabethae)、格拉汉姆巴尔通体(B.grahamii),另外2个种群与泰勒巴尔通体(B.taylorii)、文森巴尔通体阿鲁潘亚种(B.vinsonii subsp.arupensis)亲缘关系较近,有待进一步确认。结论陕西省定边县鼠疫疫区啮齿动物中有巴尔通体感染,存在人群感染的风险。

Objective To investigate the Bartonella infection and types in wild rodent populations in Dingbian plague areas of Shaanxi,and provide support for the prevention and control of Bartonella infections in humans. Methods Rodents were collected from two towns of Dingbian plague areas by trapping method. The spleens were sampled using aseptic technique and Bartonella spp were isolated and the the DNA of the suspected strains were extracted. glt A gene was amplified by PCR and sequenced,and phylogenetic analysis was carried out using Mega 6. 06 software to identify Bartonella species. Results Three species and 223 wild rodent were captured. 78 strains of Bartonella were cultivated with infection rate of 34. 98%,and the positive rate of the main rodents Meriones unguiculatus was 34. 72%. The sequence analysis suggested that the isolated Bartonella were belong to 4 strains： B. elizabethae,B. grahamii and the other two strains had the highest similarity with B. taylorii and B.vinsonii subsp. arupensis,which need further confirmation. Conclusion Bartonella infections in wild rodent populations existed in Dingbian plague areas of Shaanxi,which could pose risk of human infections.