分枝杆菌全自动染色机与人工染色的效果比较

Evaluation of the effect of auto slide stainer and manual staining by Mycobacterium

目的比较痰涂片分枝杆菌全自动染色机染色与人工染色方法之间的效果差异。方法收集疑似肺结核患者痰标本538份,同一份标本制备2张痰涂片,分别使用自动染色机和传统手工染色法进行涂片荧光染色检测。使用LED荧光显微镜进行镜检,对2种方法的涂片染色结果进行比较。计数资料采用χ~2检验,等级资料采用秩和检验。结果痰涂片自动染色机染色阳性率为5.39%(29/538),人工染色法阳性率为5.02%(27/538),二者差异无统计学意义(χ~2=0.167,P>0.05)。2种方法阳性涂片的阳性等级之间差异亦无统计学意义(UC=0.137,P>0.05)。结论痰涂片分枝杆菌全自动染色机染色与人工染色效果一致,在一定范围内可作为替代人工染色的方法进行适当推广。

Objective To evaluate the effect of auto slide stainer and manual staining by Mycobacterium the sputum smear.Methods 538 clinic sputum samples from suspected pulmonary tuberculosis patients were collected and tested. Two smears were made from the same sample and stained with fluorochrome by auto slide stainer and manual method respectively. The smears were tested by LED microscope. The differences between the two methods were compared. Results The positive rates of smear by auto slide stainer and manual method were 5. 39%（ 29/538） and 5. 02%（ 27/538）,respectively. There was no statistical significance in difference（ χ~2= 0. 167,P〉0. 05）. For the positive grates of two methods,there were also no statistical significance in difference（ UC= 0. 137,P〉0. 05）. Conclusion The effect of auto slide stainer by Mycobacterium is consistent with manual staining. As an alternative method of manual staining,auto slide stainer could be properly promoted in a certain range.