三种冷冻载杆对冷冻胚胎临床结局影响的研究

Study on clinical outcome of three different cryopreservation carriers in vitrification cryopreservation of embryo

目的探讨不同玻璃化冷冻载杆对冷冻-复苏胚胎临床结局的影响。方法分别采用自制载杆、半封闭式和封闭式商品化冷冻载杆冷冻复苏卵裂期胚胎共299个周期,观察胚胎复苏和临床妊娠情况。结果自制载杆冷冻复苏胚胎移植周期93个,复苏率、临床妊娠率和流产率分别为95.35%、52.62%和9.71%。半封闭式载杆冷冻复苏胚胎移植周期121个,复苏率、临床妊娠率和流产率分别为99.18%、56.62%和8.92%。封闭式载杆冷冻复苏胚胎移植周期85个,复苏率、临床妊娠率和流产率分别为97.38%、50.34%和9.44%。三组冷冻载杆的胚胎复苏率、临床妊娠率和流产率之间差异不显著(P>0.05)。结论三种不同冷冻载杆均能获得相近的冷冻效果。封闭式载杆在保护胚胎不受病毒感染方面有重要作用。

Objective：To explore the effects of different cryopreservation carriers on vitrification cryopreservation of human embryos. Methods：A total of 298 embryo got by fertilization in vitro and culture in vitro were collected.Human embryos were vitrification cryopreserved using different cryopreservation carriers. Pregnant outcomes were compared between the three groups. Results：The embryo recovery rates,pregnancy rate and abortion rate in self-made straw group was 95.35%,52.62% and 9.71%. The embryo recovery rates,pregnancy rate and abortion rate in opened carrier group was 99.18%,56.62% and 8.92%. The embryo recovery rates,pregnancy rate and abortion rate in closed carrier group was 97.38%,50.34% and 9.44%. there was no significant difference in the three groups（P0.05）. Conclusions：The closing cryopreservation carrier was as effective as the half-closing cryopreservation carrier and can protect embryos from crossing contamination of pathogens.