大白菜根肿病的遗传规律及抗病基因定位研究

Chinese Cabbage Root Disease Genetic Law and Positioning of Resistance Genes Research

为探究根肿病抗病遗传规律,采用根肿病抗性差异的材料G57和G70,构建了包含500个单株的F2分离群体。通过对父母本、F1及F2分离群体的人工接种表型鉴定结果进行统计分析,结果发现,材料中的根肿病抗性由显性单基因控制。为进一步定位试验材料中的抗病基因,利用678个分子标记对双亲、F1及F2群体进行了筛选验证,初步将抗病基因定位在分子标记KBRH129J18和TCR02-F。并基于2个连锁标记,开发设计了更紧密连锁的分子标记,最终获得与抗病基因连锁的5对SSR分子标记,分别为Bra0345-1、Bra0235-2、Bra0235-1、Bra19317、Bra019392。其与抗病基因的遗传距离依次为2.4,2.4,2.4,2.5,3.3 c M。开发设计的多态性标记经验证在大白菜中具有较好的适用性。

To explore the root disease resistance genetic regularity,by root disease resistance difference material,G57 and G70,the F2 segregation population containing 500 per plant was built. Through artificial inoculation of the parents,F1 and F2segregation population phenotypic characterization results were statistically analyzed. The results showed that root disease resistance of materials was controlled by a single dominant gene. In order to further localization of resistance genes in test materials,we used 678 molecular markers of parents,F1 and F2population screening verification,resistance genes was preliminarily located in molecular marker KBRH129J18 and TCR02-F.This test was based on two chain tags,chain development design closer chain of molecular markers,ultimately with resistance genes chain 5 of SSR molecular markers,respectively,Bra0345-1,Bra0235-2,Bra0235-1,Bra19317,Bra019392. Their genetic distance with resistance genes were 2. 4,2. 4,2. 4,2. 5,3. 3 c M. Validated,development and design of polymorphism markers in Chinese cabbage had good applicability.