水稻种子H_2O_2流速和种子活力的关系研究

Study on the Relationship Between H_2O_2 Velocity and Seed Vigor of Rice Seeds

研究了水稻种子H_2O_2流速与种子活力的关系,为快速无损伤鉴别水稻种子活力方法提供依据。以粳稻日本晴、籼稻金农丝苗为材料,采用高温高湿人工老化方法处理种子0,7,10 d获取不同活力水平种子,测量3种类型种子的发芽率、根长、芽长、电导率、H_2O_2含量、CAT活性等性状,并利用非损伤微测系统(NMT)测量种子的H_2O_2流速。结果表明,老化处理造成水稻种子发芽率、根长、芽长和CAT活性降低,而电导率、H_2O_2含量及外排流速增加;老化处理10 d,老化程度加重,趋势更明显。H_2O_2流速与种子发芽率表现极显著负相关的线性关系。种子的H_2O_2流速大小及方向反映了种子内部的氧化还原平衡和细胞膜的状态,通过非损伤微测技术测定H_2O_2流速可以作为水稻种子活力判断的一种方法。

In order to clarify the relationship between H2O2 velocity and seed vigor of rice seeds to provide method for rapid and noninvasive identification of rice seed vigor,germination percentage,root length,shoot length,electrical conductivity,H2O2 content,CAT activity,and H2O2 velocity of rice seeds for japonica Nipponbare and indica Jinnongsimiao after 0,7,10 d of aging times were measured. The results showed that the germination percentage,root length,shoot length and CAT activity were decreased after aging treatment,and the electrical conductivity,H2O2 content,and efflux velocity were increased. This trend was more obvious after more serious aging treatment of10 d. H2O2 flow rate and the seed germination percentage showed a significant negative correlation. The magnitude and direction of the H2O2 fluxes of the seed reflect the state of redox equilibrium and the cell membrane,H2O2 fluxes measured by the non-invasive micro-test technique may be a reliable and sensitive method to evaluate seed germination and vigor.