沉默Yes相关蛋白1对人非小细胞肺癌细胞增殖和侵袭的影响

Effect of silencing of Yes-associated protein 1gene on proliferation and invasion of human non-small cell lung cancer cells

目的研究Yes相关蛋白1(YAP1)在非小细胞肺癌细胞增殖、细胞周期、迁移、侵袭中的作用。方法通过慢病毒介导的小干扰RNA靶向抑制人非小细胞肺癌细胞株A549中YAP1基因的表达,采用CCK-8法、流式细胞术分别检测沉默YAP1对A549细胞增殖及凋亡周期的影响,Transwell实验观察沉默YAP1对A549细胞迁移、侵袭能力的影响。结果沉默YAP1后,A549细胞YAP1mRNA和蛋白表达均下调(P<0.01),CCK-8实验结果显示沉默YAP1抑制A549的增殖、增加G0/G1期的细胞比例(P<0.01),Transwell实验结果示沉默YAP1抑制A549细胞的迁移、侵袭(P<0.01)。结论沉默YAP1基因对非小细胞肺癌细胞的恶性生物学特征具有抑制作用,YAP1可能成为肺癌治疗的潜在靶标。

Objective To explore the role of Yes-associated protein 1（YAP1）in the proliferation,cell cycle,migration and invasion of human non-small cell lung cancer（NSCLC）cells.Methods A lenti-siRNA targeting YAP1（si-YAP1）was used to inhibit the expression of YAP1 gene of human NSCLC cell line A549 cells.CCK-8assay and flow cytometry were used to determine the effects of silencing of YAP1 expression on A549 cells proliferation and cell cycle,respectively;Transwell assay was used to observe the effect of silencing of YAP1 expression on A549 cell migration and invasion.Results After infection with si-YAP1,the expressions of YAP1 mRNA and protein in A549 cells were significantly down-regulated（P〈0.01）.YAP1 silencing significantly inhibited A549 cell proliferation,increased the percentage of cells in G0/G1 phase（P〈0.01）,and significantly decreased the migration and invasion of A549cells（P〈0.01）.Conclusion YAP1 silencing can inhibit malignant biological characteristics of NSCLC,which suggests that YAP1 gene may serve as an important target in the gene therapy of lung cancer.