摘要
目的探讨长链非编码RNA（LncRNA）GAS8-AS1在甲状腺微小乳头状癌中的表达变化特点及临床意义。
方法收集70例甲状腺微小乳头状癌（PTMC）患者及24例良性结节患者的新鲜组织标本，采用实时荧光定量PCR（RT-qPCR）技术检测GAS8-AS1的表达差异，分析其表达水平与临床病理特征的相关性。
结果GAS8-AS1在PTMC组织中表达与良性结节及癌旁组织比较均明显下降（P〈0.05），且癌组织中GAS8-AS1的降低程度与颈部中央区淋巴结转移相关（P〈0.05），多因素分析中GAS8-AS1仍为PTMC患者出现颈部中央区淋巴结转移的独立危险因素，GAS8-AS1鉴别PTMC患者是否出现淋巴结转移的ROC曲线下面积为0.7173（P〈0.05）。
结论GAS8-AS1有可能成为PTMC诊断及淋巴结转移预测的潜在分子标志物。
ObjectiveTo investigate the expression level and clinical significance of long non-coding RNA（LncRNA） growth arrest specific gene-antisense 1（GAS8-AS1） in papillary thyroid microcarcinoma（PTMC） patients.MethodsWe investigated the expression profile of GAS8-AS1 in tissue samples of patients with PTMC as well as nodular goiter（NG） by quantitative real-time polymerase chain reaction（RT-qPCR）.ResultsGAS8-AS1 in cancer tissue was down-regulated in PTMC patients compared with adjacent thyroid tissue and NG samples（P〈0.05）. Lower level of GAS8-AS1 was also correlated with central cervical lymph node metastasis（CLNM, P〈0.05）. The area under the ROC curve for GAS8-AS1 was up to 0.717 3 in CLNM prediction（P〈0.05）.ConclusionGAS8-AS1 may act as a potential biomarker for PTC diagnosis and CLNM prediction.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2017年第8期687-692,共6页
Chinese Journal of Endocrinology and Metabolism
基金
北京市自然科学基金重点课题（7141002）
