HLA抗体的产生与孕妇妊娠次数关系的研究

Relationship between HLA antibodies produceand the number of gravidity

目的研究HLA抗体的产生与孕妇妊娠次数的关系。方法选择2016年11月9日-2017年3月31日在邵逸夫医院下沙院区登记待产的24-28周856例孕妇为研究对象,收集其外周血血样,利用单克隆抗体固相血小板抗体实验检测孕妇血清中HLA抗体的表达情况。结果共检出HLA抗体阳性孕妇67例,抗体阳性率为7.8%。334例首次妊娠的孕妇中,有15例HLA阳性的,阳性率为4.5%;216例无流产史的两次或以上妊娠的孕妇中,有14例阳性,阳性率为6.5%;107例有一次流产史的孕妇中,有10例阳性,阳性率为9.3%;223例有两次及以上流产史的孕妇中,有28例阳性的,阳性率为12.6%。妊娠次数与HLA抗体发生率呈正相关。结论二次及以上妊娠的孕妇HLA抗体发生率高于首次妊娠的孕妇。

Objective Study the relationship between HLA antibodies production andthe number of Gravidity. Methods A total of 856 pregnant women of first pretanal in Xiasha Hospital of Sir Run Run Shaw Hospital of Zhejiang University from Nov 2016 to Mar 2017 were recruited.The blood samples were collected during 24-28 weeks of pregnancy, and the expression of platelet-specific alloantibody was detected by solid-phase red cell adherence assay. Results There were 67 posi- tive HLA antibody in pregnant women, and the antibody positive rate was 7.8%.There were 15 positive HLA antibody in 334 first pregnan! women, 14 in 214 two or more pregnancy pregnant women without abortion, 10 in 107 pregnant women with once abortion, 28 in 223 pregnant women with two or more abortion.The antibody positive rate respectively is 4.5%, 6.5%, 9.3% and 12.6%.The production of HLA antibody was significantly associated with the numberof gravidity.Conclusion The antibody positive rate of two or more pregnancy is significantly higher than the fisrt pregnancy.