3种方法检测O型血孕妇IgG血型抗体效价分析

Analysis of antibody potency of IgG blood group in O-type blood pregnant women by three methods

目的应用微柱凝胶试剂卡、微柱玻璃珠试剂卡、经典试管法同时检测夫妇ABO血型不合的O型孕妇IgG血型抗体效价,探讨微柱凝胶试剂卡、微柱玻璃珠试剂卡检测孕妇血型抗体效价的界值。方法用微柱凝胶试剂卡、微柱玻璃珠试剂卡、经典试管法平行检测O型血孕妇Ig G血型抗体效价。结果微柱凝胶试剂卡、微柱玻璃珠试剂卡以1∶128为界值与试管法1∶64比较,阳性检出率差异无统计学意义(P>0.05)。结论使用微柱凝胶试剂卡、微柱玻璃珠试剂卡检测O型孕妇IgG血型抗体效价的临床参考值应设为≥1∶128。

Objective To investigate the effect of microcolumn gel reagent card, micro-column glass bead reagent card and classical test tube on the antibody titer of O-type pregnant women with ABO blood group. Determination of antibody po- tency of pregnant women with rabbit by reagent card. Methods Using mieroeolumn gel reagent card, micro-column glass beads reagent card, and classical test tube parallel detection to detect the IgG blood group antibody titer of O blood type preg- nant women. Results There was no significant difference in the positive detection rate between the microcolumn gel reagent card and the microeolumn glass beads reagent card at 1：128 and the test tube method at 1：64 （P〉0.05）. Conclusion The clinical reference value of microeolumn gel reagent card and mierocolumn glass beads reagent kit for detecting antibody titer of IgG blood group in O-type pregnant women should be set to ≥ 1 ： 128.