添加羧化壳聚糖采血后即刻分离制备血小板可行性研究

Study on theimmediate separation feasibility of PLT preparation by adding to carboxylation chitosan

目的探讨使用甲壳素衍生物—羧化壳聚糖即刻分离制备血小板的可行性,寻找血液成分分离新方向。方法随机选取大连地区献血者40名,当日成功献血时同步采集全血10 m L,分成实验组,自然沉降对照组和离心对照组进行实验。从阿拉丁试剂公司购买水溶性壳聚糖,用血液保存液Ⅱ配制成(0.25~2.0)mg/m L不同浓度溶液,按1∶4比例加入到2 m L全血中,静置4 h,观察血液分离速度,计数血浆中红细胞,白细胞及血小板,观察血小板聚集现象及红细胞形态变化;在最适壳聚糖添加浓度的红细胞中加入适量红细胞保存液予以4℃保存,35 d保存期内检测溶血率。结果加入1.0~2.0 mg/m L浓度壳聚糖的实验组血液分离速度均明显快于自然沉降对照组,血浆中均仅存微量红细胞,富血小板,35d时无明显溶血,同离心对照组。结论壳聚糖可以用于采血后即刻分离富血小板血浆。

Objective To investigate the use of chitin derivatives-carboxylation chitosan immediate separation feasibility of PLT preparation , and to look for a new direction in separation feasibility of blood components. Methods 40 samples of blood donors were divided into the experimental group, natural sedimentation control group and centrifugal control group randomLy in Dalian. 2 mL of whole blood were mixed with differ-ent concentrations of carboxylation chitosan which were diluted by blood preservation solution 1I by according to the ratio of 1 ：4. plasma precipitation amount were surveyed after 4 hours , with numbers of red blood cells, white blood cell and platelet , PLT aggregation and the changes of red blood cell morphology were observed. Finally, suitable amount of MAP were added into the optimal chitosan in preservation,and hemolysis of red blood cells were tested in 35 days. Results Suitable amount of chitin experimental group blood sedimentation rate were significantly faster than static device control group, and plasma remaining trace red blood cells, PLT-rich , and 35 days no obvious hemolysis. Conclusion carboxylation chitosan could be used in PLT preparation.