高脂血症痰证动物模型实验研究及思考

Experimental Study on Animal Model of Phlegm Syndrome of Hyperlipidemia and Further Thinking

目的:采用高脂饲料喂养建立SD大鼠高脂血症痰证动物模型。方法:第1次实验将30只8周龄SD雄性大鼠随机分为正常组10只及模型组20只,分别给予正常饲料和高脂饲料喂养,自由饮食及饮水,于第4、6、8周末,检测血清胆固醇(TC)、总甘油三酯(TG)、低密度脂蛋白(LDL-C)及高密度脂蛋白(HDL-C)水平,8周末采血后处死动物并取出完整肝脏,称量湿重后做HE染色病理切片。第2次实验直接将8只正常组和8只模型组大鼠分别喂养8周,8周末检测大鼠的血脂情况,并将两次实验8周末血脂水平进行比较。实验过程中对大鼠的一般情况进行观察并记录。结果:第1次实验中模型组大鼠与正常组相比,第4、6周末TC升高,差异有统计学意义(P<0.05),其余3项无统计学差异,8周末血脂四项与正常组相比均无差异,模型组肝脏病理切片有明显脂肪样变,两组主动脉切片均无明显异常;第2次实验第8周末,模型组大鼠TC、TG升高,HDL-C下降,差异有统计学意义(P<0.05),LDL-C无差异。两次实验的结果具有显著差异(P<0.05)。两次实验模型组均出现了体胖、倦怠、饮食饮水减少的情况,初步判断符合痰证的证候特点。结论:采用高脂饲料喂养SD大鼠能初步建立高脂血症痰证动物模型,但其造模时间的长短及痰证证候的判断还有待进一步探索和完善,所造动物模型的稳定性和可重复性需要进一步探究。

Objective： To establish animal model of phlegm syndrome of hyperlipidemia on SD rats by high-fat diet.Method： In the first experiment,30 8-week-old male SD rats were randomly divided into normal group（ n = 10） and model group（ n = 20）,giving normal food and high-fat diet in part,free eating and drinking. At the end of 4th,6th,8th week,the levels of serum total cholesterol（ TC）,triglyceride（ TG）,low density lipoprotein（ LDL-C） and high density lipoprotein cholesterol（ HDL-C） were measured,respectively. And at the end of 8th week,after blood was collected,the animals were sacrificed and their intact livers were removed. Weigh wet weight and then make pathological sections with hematoxylin and eosin stained. In the second experiment,8 male SD rats in normal group and 8 in model group were fed for eight weeks. The blood lipid of rats was measured at the end of 8th week and the result was compared with that of the first experiment. The general behaviors and conditions of rats were observed and recorded during the experiment. Result： In the first experiment,compared with normal group,the serum level of TC at the end of 4th and 6th week of model group increased with significant difference（ P〈0. 05）. But the serum levels of TG,LDL-C and HDL-C remained no statistical difference. At the end of 8th week,all lipid levels showed no difference compared with the normal group. The liver pathological section of model group rat had obvious fatty degeneration compared with normal group,and two groups＇ aortic sections showed no obvious abnormality. In the second experiment,the serum levels of TC,TG increased and HDL-C decreased. The difference was statistically significant（ P〈0. 05）. The results of the two experiments were significantly different（ P〈0. 05）. However,the two models groups both showed poor appetite for food and water,lassitude,and lower body weight,compared with normal group. Conclusion： The phlegm syndrome of hyperlipidemia animal model can be preliminarily established with high-fat diet fed SD rats,but the length of feed time and eval-uation of phlegm syndrome remains to be further explored,and the stability and repeatability of the animal model require further exploration.