基于蛋白质组学和代谢组学的HIV/AIDS肺脾气虚证的研究

Based on Proteomics and Metabolomics of HIV/AIDS Deficiency Syndrome of Lung-spleen Qi Patients

现在已经进入了系统生物学高速发展的时代了,蛋白组学和代谢组学作为系统生物学的组成部分,对中医证候本质的研究将会更加客观化、标准化。采用i TRAQ-MS技术对HIV/AIDS肺脾气虚证患者血清进行了蛋白质组学检测,发现差异表达蛋白APOA-II、APOB、AZGP1;采用LC-MS技术对HIV/AIDS肺脾气虚证患者尿液和血液进行了代谢组学分析,比对出了9种可能的潜在标志物,包括TG、天冬氨酸、葡萄糖、磷脂酰乙醇胺等。对蛋白质组和代谢组学结果进行探讨,以期寻找HIV/AIDS肺脾气虚证的物质基础,为证候诊断提供量化依据。

Now we have entered the era with rapid development of systems biology,proteomics and metabonomics.As a part of systems biology,the research of syndrome essence of traditional Chinese medicine would be more objective and standardized. Research using i TRAQ MS technology to HIV/AIDS patients with temper deficiency syndrome serum proteomics is investigated,finding out the differentially expressed proteins APOA-II,APOB,AZGP1. Use LC-MS technique for HIV/AIDS patients with spleen deficiency and analyze blood urine metabonomics. Compare the nine possible potential markers,including TG,aspartic acid,glucose,phosphatidyl ethanolamine,etc. This paper discusses the results of proteomics and metabonomics in order to find out the material basis for the HIV/AIDS lung and spleen deficiency and provide the basis for objective.