穴位埋线联合东莨菪碱治疗对吗啡依赖大鼠十二指肠表达胃泌素和生长抑素的影响

Effects of Acupoint Catgut Embedding Therapy Combined Scopolamine on Levels of Gastrin and Somatostatin of Duodenum in Morphine Dependent Rats

目的:探讨穴位埋线加东莨胆碱治疗对吗啡依赖大鼠十二指肠胃泌素(Gas)、生长抑素(SS)免疫反应(IR)细胞的影响。方法:将大鼠大鼠随机分为正常组、自然戒断组(简称戒断组)、东莨菪碱治疗组(简称西药组)、穴位埋线治疗组(简称穴位埋线组)及穴位埋线加东莨菪碱治疗组(简称穴位埋线加西药组)。经腹腔注射盐酸吗啡建立吗啡依赖大鼠模型,采用不同的方法干预,观察戒断症状,分别于干预后7 d和14 d处死取材,观察十二指肠黏膜Gas和SS-IR形态特点,并比较各组十二指肠组织匀浆中Gas和SS的含量。结果:吗啡依赖大鼠戒断症状明显,其十二指肠黏膜Gas和SS-IR细胞数量明显增加(P<0.05),组织匀浆中Gas和SS含量明显增加(P<0.01);治疗7 d后,穴位埋线加西药组各指标明显改善,与正常组无显著差异(P>0.05),治疗时间延长至14 d,西药组与穴位埋线组各项指标与正常组接近(P>0.05)。结论:西药治疗与穴位埋线治疗对吗啡依赖所致大鼠的戒断症状及十二指肠分泌Gas和SS的功能均有逆转作用,两种方法结合可缩短治疗时间,治疗效果更佳。

Objective： To observe the effects of acupoint catgut embedding therapy combined western medicine scopolamine on the levels of gastrin（ Gas） and somatostatin（ SS） of duodenum in morphine dependent rats. Methods： The rats were randomized into control group,spontaneous withdrawal group,scopolamine group,acupoint catgut embedding group,and acupoint catgut embedding combined scopolamine group. Morphine dependent rat model was induced by administration（ ip） of morphine. Withdrawal syndromes were evaluated after different treatments. Rats were sacrificed after treated for 7 and 14 days respectively. Compare morphological changes,Gas and SS levels of duodenum among different groups. Results： Withdrawal syndromes appeared in morphine dependent model group obviously with increased Gas and SS-IR expression in duodenum and tissue homogenate. All detecting items of acupoint catgut embedding combined scopolamine group were improved significantly after treated for 7 days and there was no significant difference compared to those of the control group（ P〉0. 05）. Detecting items of scopolamine group were close to the those of the control group after treated for 14 days（ P〉0. 05）. Conclusion： Both western medicine scopolamine and acupoint catgut embedding therapy could reverse withdrawal syndromes,Gas and SS levels of duodenum in morphine dependent rats. However,combined two therapies could shorten the time of treatment with better effect.