3D水凝胶压应力模型建立及压应力对成骨细胞的影响

3D Hydrogel Compression Model and Effect of Cyclic Compression on Osteoblast Differentiation

目的:通过建立3D水凝胶细胞模型,采用不同强度、频率、时间对MC3T3-E1细胞进行机械加压干预,进而探讨促进成骨细胞生长的适宜压应力方案。方法:设计不同强度、频率、时间的压应力梯度方案对成骨细胞MC3T3-E1进行干预。加压干预结束后即刻收集细胞,对样本中的ATF4、ALP、Runx2、Osteocalcin、RANKL和RANK mRNA进行定量检测。结果:经统计学分析后发现不同强度和频率对ALP(P<0.05)、Runx2(P<0.01)交互作用显著。此外,4h加压Runx2的表达量比12h加压的高(P<0.05);4h加压RANKL的表达量比12h加压的低(P<0.05)。结论:确定了压力强度和频率后,对3D细胞水凝胶模型施加一段时间的压应力发现,以1%强度、0.5Hz频率、4h的压应力干预方案能够促进成骨细胞系的生长。

Objective： 3D hydrogel cell model was established,and cyclic compressive loading on MC3T3-E1 cell with different intensities,frequencies and durations was applied,in order to research the suitable solution about promoting the osteoblast differentiation with cyclic compression. Methods： Cyclic compressive loading on MC3T3-E1 cell was applied with different intensity,frequency and time. After compressive loading finished,the total RNA extraction from cell-gel constructs were performed and quantified ATF4,ALP,Runx2,Osteocalcin,RANKL and RANK mRNA. Results： RANKL and RANK mRNA expression significantly with different frequencies cyclic compressive loading（ P 〈0. 05）,and ALP mRNA（ P 〈0. 05） and Runx2 mRNA（ P 〈0. 01） expression significantly with different intensities and frequencies cyclic compressive loading（ P 〈0. 05）. Meanwhile,Runx2 mRNA expression with 4h significant higher than 12h（ P 〈0. 05）,and RANKL mRNA expression with 4h significant lower than 12h（ P 〈0. 05）. Conclusion： Determine the stress intensity and frequency,1% intensity,frequency of 0. 5 Hz,4 h of cyclic compression intervention could promote the growth of osteoblasts-like cells in the 3D hydrogel model.