气囊对腹壁撤退反射实验结果的影响

Effect of air bag on the outcome of abdominal withdrawal reflex test

目的为腹壁撤退反射实验优选出恰当的制备气囊的材料。方法分别通过数显游标卡尺及X光图像对不同气囊在不同压力值下的直径进行测量;并采用3分疼痛阈值作为判断标准,测试不同气囊对大鼠造成明显内脏疼痛时所对应的压力值。结果圆形乳胶气球的直径随压力值增加变化均匀和缓,并且可以满足实验中常用的压力范围(20~80 mm Hg),其对大鼠造成内脏疼痛的阈值在伤害性刺激值附近,不会对大鼠肠道造成不可逆的损伤。结论腹壁撤退反射实验中使用圆形乳胶气球制备气囊较为理想。

Objective To optimize the preparation of air bag materials for abdominal withdrawal reflex test.Methods To measure the diameters of different pressue-air bags by digital vernier caliper and X ray imaging. Using the pain threshold（ 3 points） as a criterion to test the pressure values of different air bags in the rats with corresponding visceral pain sensitivity. Results With the increasing pressure,the changes of the diameter of round latex balloon were uniform and gentle,and could meet the pressure range used in the experiment（ 20 ～ 80 mm Hg）. The threshold of visceral pain in rats was near the nociceptive stimulus value,and would not cause irreversible damage to the rat intestinal tract. Conclusions Air bag made from round latrex ballon is an ideal tool to be used in abdominal withdrawal reflex test in rats.