超声造影定量参数与肝癌细胞增殖活力、病灶内血管新生的相关性研究

The correlation of the quantitative ultrasonography parameters with the liver cancer cell proliferation activity and intralesional angiogenesis

目的:研究超声造影定量参数与肝癌细胞增殖活力、病灶内血管新生的相关性。方法:选择接受手术切除的46例肝癌患者、同期在因腹部外伤接受肝脏部分切除术的28例患者,肝癌患者接受超声造影并计算定量参数;收集肝癌患者的肝癌组织、癌旁组织以及腹部外伤患者的正常肝脏组织,检测增殖基因、抑癌基因、血管新生基因的表达量。结果:肝癌组织的IMAX显著高于癌旁组织,TTP、WT、mTT显著短于癌旁组织;肝癌组织、癌旁组织中RNF180、Merlin、PICK1、PTEN的蛋白表达量均显著低于正常肝脏组织且与肝癌组织IMAX呈负相关,与肝癌组织TTP、WT、mTT呈正相关;肝癌组织、癌旁组织中EphB4、SALL4、Cripto-1、VEGF、PDGF、EGFR、Ang-2的蛋白表达量均显著高于正常肝脏组织且与肝癌组织IMAX呈正相关,与肝癌组织TTP、WT、mTT呈负相关。结论:肝癌病灶超声造影定量参数的改变对细胞增殖、血管新生具有评估价值。

Objective：To study the correlation of the quantitative ultrasonography parameters with liver cancer cell proliferation activity and intralesional angiogenesis.Methods：A total of 46 patients with liver cancer who received surgical resection in Hancheng People＇s Hospital as well as 28 patients who received partial hepatectomy for abdominal trauma during the same period were selected,the patients with liver cancer received ultrasonography,and the quantitative parameters were calculated;the liver cancer tissue and adjacent tissue of patients with liver cancer as well as the normal liver tissue of patients with abdominal trauma were collected to determine the expression of proliferation genes,tumor suppressor genes and angiogenesis genes.Results：The IMAX of liver cancer tissue was significantly higher than that of adjacent tissue while the TTP,WT and mTT were significantly shorter than those of adjacent tissue;RNF180,Merlin,PICK1 and PTEN protein expression in liver cancer tissue and adjacent tissue were significantly lower than those in normal liver tissue,negatively correlated with the IMAX of liver cancer tissue,and positively correlated with the TTP,WT and mTT of liver cancer tissue;EphB4,SALL4,Cripto-1,VEGF,PDGF,EGFR and Ang-2protein expression in liver cancer tissue and adjacent tissue were significantly higher than those in normal liver tissue,positively correlated with the IMAX of liver cancer tissue,and negatively correlated with the TTP,WT and mTT of liver cancer tissue.Conclusions：The changes in the quantitative parameters of liver cancer ultrasonography are valuable for evaluating the cell proliferation and angiogenesis.