基于3.0 T磁共振成像系统初步观察WHBE兔脑部形态解剖结构

Preliminary observation of the anatomical structures of the brain in WHBE rabbits by 3.0 T magnetic resonance imaging system

目的基于3.0 T磁共振成像(MRI)技术,活体观察WHBE兔脑部形态解剖结构,累积WHBE兔脑的影像学基本生物学数据,为进一步拓宽WHBE兔的应用提供背景资料。方法成年雄性WHBE兔9只,用3%戊巴比妥钠溶液静脉注射麻醉,行3.0 T MRI加兔脑专用线圈常规横断面和矢状面扫描,并测量分析脑部的各种结构大小。结果 MRI扫描可成功获得活体WHBE兔脑矢状面和横断面T2WI或T1WI图像,可完整清晰地观察到活体WHBE兔的嗅球、大脑、小脑和垂体等基本结构,并发现了4只兔脑T2WI中左右两侧颞叶海马区高信号,相应区域在T1WI出现低信号外,其余均无异常。同时,获得了额叶、海马、大脑、侧脑室、垂体等相关解剖结构的参考值。结论采用3.0 T磁共振成像系统加上兔脑专用线圈,可以清晰地观察到WHBE兔脑部的各种形态解剖结构,并初步建立了WBHE兔脑影像学基本数据。

Objective To observe the morphological structures of WHBE rabbit brain in vivo based on 3. 0 T magnetic resonance imaging system（ MRI）,accumulate the basic biological data of WHBE rabbit brain imaging,and provide a background information to further expand the WHBE rabbit application. Methods Nine healthy adult male WHBE rabbits were intravenously anesthetized with 3% pentobarbital sodium. 3. 0 T MRI plus rabbit brain dedicated coil was used to perform routine transverse and sagittal scans,and the size of brain structures were measured. Results MRI scanning can be successfully performed to obtain sagittal and transverse T2WI or T1WI images of WHBE rabbit brain in vivo,and can be clearly observed the basic structures of WHBE rabbit brains in vivo,such as olfactory bulb,cerebrum,cerebellum and pituitary gland. In addition,high signal was found in the hippocampus of the left and right temporal lobes in 4 rabbits with T2WI,but also low signal appeared in the corresponding regions in T1WI,and the others were not abnormal. Mean-while,the reference data of frontal lobe,hippocampus,cerebrum,lateral ventricles,pituitary gland and other related anatomical structures were also obtained. Conclusions Using the 3. 0 T magnetic resonance imaging system and rabbit brain coil,the morphological and anatomical structures of rabbit brain can be clearly observed,and the basic imaging data of WHBE rabbits brain have been established preliminarily.