维药阿育魏果中总黄酮提取工艺及含量测定

Determination of Extraction Process of Total Flavonoids in Seed of Trachyspermum ammi (L.) Sprague of Uyghur Crude Drug

[目的]探讨阿育魏果中总黄酮的提取方法及含量测定方法。[方法]采用正交试验,以提取的总黄酮的含量为评价指标,紫外可见分光光度法测定总黄酮含量。[结果]超声法提取阿育魏果中总黄酮的含量为10.378%,相对标准偏差为2.38%(n=3)。最优工艺如下:时间为15 min,功率为150 W,料液比为1∶20,乙醇浓度为70%。上述4个因素对阿育魏果中总黄酮提取率的影响从大到小依次为乙醇浓度、料液比、超声功率和超声时间。[结论]超声提取阿育魏果中总黄酮快速且高效;紫外外分光光度法准确度较高,偏差较小。

[Objective] The aim was to study the methods of extraction and determination of total flavonoids in seed of Trachyspermum ammi （L.） Sprague. [ Method ] Through orthogonal experiments and taking total flavonoids extracted as the evaluation index, UV-Vis speetrophotometry method was used to determine the optimum technological conditions of total flavonoids extraction in seed of Trachyspermum ammi （ L. ） Sprague. [ Result] The total flavonoids content measured in seed of Trachyspermum ammi （L.） Sprague was 10. 378% ,and the relative standard deviation was 2.38% （n = 3 ）. The best ultrasonic extraction technology of total flavonoids was as followed：the ultrasonic time was 15 min; the ultrasonic power was 150 W; the solid-liquid ratio was 1： 20; and the concentration of ethanol was 70%. The effects of the four factors was in order of etha- nol concentration,solid-liquid ratio,ultrasonic power and ultrasonic time. [ Conclusion ] The ultrasonic extraction techniques of total flavonoids from seed of Trachyspermum ammi （L.） Sprague are quick and efficien. The UV-Vis spectrophotometry method is accurate and reliable.