大黄素对高糖诱导的大鼠肾小球系膜细胞TGF-β1/ERK1/2及FN蛋白表达的影响

Effects of emodin on TGF-β1/ERK1/2 and FN protein expression in rat glomerular mesangial cells induced by high glucose

目的探讨大黄素（emodin，EMO）对高糖诱导的大鼠肾小球系膜细胞（glomerular mesangial cells，MCs）转化生长因子-β1（TGF-β1）/细胞外信号调节激酶1/2（ERK1/2）及细胞外基质（extracellular matrix，ECM）FN蛋白表达的影响。方法将常规培养的MCs分为正常组（NG，5.56 mmol/L葡萄糖）、 甘露醇组（MA，5.56 mmol/L葡萄糖＋24.44 mmol/L甘露醇）、溶剂组（0.1% DMSO＋30 mmol/L葡萄糖）、高糖组（HG，30 mmol/L葡萄糖）及大黄素干预组（1 μmol/L、10 μmol/L EMO＋30 mmol/L葡萄糖）。CCK-8检测细胞增殖情况；Western blotting检测各组细胞TGF-β1、p-ERK1/2、FN蛋白表达。结果与正常组比较，高糖培养48 h MCs增殖显著（P〈0.05，P〈0.01），p-ERK1/2、TGF-β1、FN蛋白表达明显增高（P〈0.05）；与高糖组比较，大黄素干预组能抑制MCs增殖，并显著降低p-ERK1/2、TGF-β1、FN蛋白表达（P〈0.05或P〈0.01）。结论大黄素能抑制高糖诱导下的MCs细胞增殖，下调TGF-β1、p-ERK1/2、FN蛋白表达，从而发挥对糖尿病肾病（diabetic nephropathy，DN）的防治作用。

ObjectiveTo investigate the effects of emodin （EMO） on the TGF-β1/ERK1/2, extracellular matrix （ECM） and FN protein in rat glomerular mesangial cells （MCs） induced by high glucose. MethodsMCs were divided into four groups： a normal control group （NG, 5.56 mmol/L glucose）, a mannitol group （MA, 5.56 mmol/L＋24.44 mmol/L mannitol）, a solvent group （0.1%DMSO＋30 mmol/L glucose）, a high glucose group （HG, 30 mmol/L glucose） and an Emodin intervention group （1 μmol/L, 10 μmol/L EMO＋30 mmol/L glucose）. The cell proliferation was measured by CCK-8 assay. The levels of TGF-β1, p-ERK1/2 and FN were detected by Western blotting. ResultsCompared with the NG group, the HG group presented obvious proliferation after cultivation for 48 h （P〈0.05 or P〈0.01） and produced significantly increased levels of p-ERK1/2, TGF-β1 and FN （P〈0.05）. Compared with the HG group, the Emodin intervention group showed suppressed proliferation and remarkably reduced levels of p-ERK1/2, TGF-β1, and FN （P〈0.05 or P〈0.01）. ConclusionsEmodin can inhibit the proliferation of MCs induced by high glucose and down-regulate the levels of p-ERK1/2, TGF-β1 and FN, which plays a role in the prevention and treatment of diabetic nephropathy.