依托咪酯对小鼠胃肠推进运动的影响

Effects of etomidate on the propulsive gastrointestinal motility in mice

目的观察依托咪酯（etomidate，Eto）对小鼠胃肠推进运动的影响。方法将昆明种小鼠随机分为4组（n=10）：生理盐水（Ns）组、依托咪酯5mg·kg-1、10mg·kg-1、20mg·kg-1（Eto5、Eto10、Eto20）组。各组腹腔注射NS或相应剂量的依托咪酯后，立即用0．2ml炭末悬浊液灌胃，40min后将小鼠处死，以幽门为起点测量炭末悬浊液在小肠内的移动距离（小肠炭末移动距离）和小肠全长，并计算小肠推进率。结果与NS组相比，Eto10组、Eto20组小肠炭末移动距离均明显缩短（P〈0．01），小肠推进率均明显降低（P〈0．01）。与Eto5组相比，Eto10组、Eto20组小肠炭末移动距离均缩短（P〈0．05，P〈0．01），小肠推进率均降低（P〈0．05，P〈0．01）。与Eto10组相比，Eto2020组小肠炭末移动距离缩短（P〈0．05），小肠推进率降低（P〈0．05）。结论依托咪酯可抑制小鼠的胃肠推进运动，且随着剂量的增加，抑制作用增强。

Objective To investigate the effects of etomidate on the propulsive gastrointestinal motility in mice. Methods Kunming mice were randomly divided into four groups （ n = 10） ： a normal saline （NS） group, and etomidate 5 mg · kg-1, 10 mg · kg-1 and 20mg·kg-1（Eto5, Eto10 and Eto20） groups. Each mice was intraperitoneally injected with NS or corresponding doses of etomidate before intragastrical administration of 0.2 ml carbon suspension. Then, 40 min later, the mice were sacrificed. The distance that the suspension was pushed in the small intestine and the total length of the small intestine were measured starting from the pylorus, and the propulsion rate of the small intestine was calculated. Results Compared with the NS group, obviously decrease in the moving distance of carbon in the small in- testine and the propulsion rate of the small intestine were found in the Etol0 group and Eto20 groups （ P 〈 0.01 ）. Com- pared with the Eto5 group, obviously decrease in the moving distance of carbon in the small intestine and the propulsion rate of the small intestine were found in the Eto10 group and Eto20 groups （ P 〈 0.05 or P 〈 0.01 ）. Compared with the Eto10 group, obviously decrease in the moving distance of carbon in the small intestine and the propulsion rate of the small intestine were found in the Eto20 group （P 〈0. 05 ）. Conclusions Etomidate can inhibit the propulsive gastroin- testinal motility in mice, and the inhibitory effects are enhanced as the dose used increases.