apelin-APJ系统基因多态性与大动脉僵硬度相关性研究

Association of apelin-APJ pathway single nucleotide polymorphisms and arteriosclerosis

目的探讨福建沿海地区高血压人群apelin及其受体APJ基因的单核苷酸多态性(SNPs)与臂踝脉搏波传导速度(baPWV)反应的大动脉僵硬度相关性。方法以2011年福建沿海地区的横断面调查为基础,纳入556例高血压患者,其中男性173例,女性383例,年龄(58±11)岁,均进行体格检查、实验室检查,及动脉硬化检测仪测baPWV及采用Taqman@MGB探针法获得apelin基因rs56204867、rs3761581、rs3115757和APJ基因rs9943582、rs7119375共5个SNPs的基因分型。结果 556例高血压患者中,动脉硬化组423例的baPWV值为(1 764±296)mm/s,正常组133例的baPWV值为(1 266±111)mm/s,认为baPWV≥1 400mm/s为动脉硬化;无论男女,动脉硬化组与正常组间5个SNPs相应的等位基因分布差异无统计学意义(P均>0.05);调整年龄、协方差分析提示男性apelin基因rs3115757-C突变等位基因携带者baPWV明显快于rs3115757-G野生等位基因携带者[(1 536±375)mm/s vs(1 383±304)mm/s,P=0.01)]。二元logistic回归分析示,女性高血压人群中携带apelin基因rs56204867-C的动脉硬化风险增加(OR=2.087,95%CI:1.018~4.276,P=0.04)。结论女性高血压人群apelin基因rs56204867与其大动脉弹性下降有关,提示调查地区人群apelin-APJ系统基因多态性与高血压血管损害有关。

Objectives To investigate the association of apelin-APJ patyway single nucleotide and arteriosclerosis through brachial ankle pulse wave velocity（baPWV）.Methods A total of 556 subjects,173men and 383 women,were enrolled in this study based on a cross-sectional survey in Fujian coastal area in 2011.All subjects were given physical examination,laboratory test and baPWV measured using the PWV device.Five single nucleotide polymorphisms（SNPs）were evaluated using Taqman@MGB probe,including apelin rs56204867,apelin rs3761581,apelin rs3115757,APJ rs9943582 and APJ rs7119375.ResultsA total of 556 hypertension subjects,423 cases in arteriosclerosis group,the baPWV were（1 764±296）mm sand（1 266±111）mm/sin 133 formal cases.There was no significant difference in the distribution of alleles between the atherosclerosis group and the normal group of 5SNPs（all P〈0.05）.The male subjects with mutant allele of apelin rs3115757-C had a significantly higher baPWV than those with wild allele after adjusting for age（P〈0.05）.The female subjects with mutant allele of apelin rs56204867-C had an increased risk of developing abnormal baPWV（OR=2.087,P〈0.05）.Conclusion The apelin SNP rs56204867 is associated with reduced arterial elasticity in female subjects with hypertension.The SNPs in apelin-APJ pathway may be associated with hypertension and hypertensive vascular damage.