小鼠胰腺炎模型中胰腺导管腺体与导管上皮修复再生的研究

Study on the relationship between PDG and pancreatic ductal epithelium regeneration in mouse model of pancreatitis

目的:探讨在炎症情况下胰腺导管腺体(PDG)与胰腺导管上皮修复再生过程的关系。方法:采用病理学评分评估雨蛙肽诱导急慢性胰腺炎小鼠模型的建立,采用病理组织学方法观察胰腺组织中PDG及导管结构病理学变化;免疫组化检测PDG及胰腺导管上皮细胞中Pdx1、Sox9、Ngn3的表达情况,利用免疫组化评分(HIS评分)评估其阳性表达率。结果:成功建立小鼠急慢性胰腺炎模型,其病理学评分分别为3.33±0.52和8.50±0.55,与对照组相比差异有统计学意义;在慢性胰腺炎模型中,PDG结构出现明显的增殖变化,且Pdx1、Sox9、Ngn3的表达均有阳性,HIS评分分别为1.966 7±0.233 8、0.700 0±0.209 8、0.900 0±0.209 8,与对照组相比差异有统计学意义。结论:PDG结构与胰腺导管上皮组织的修复再生相关,且与成体胰腺的分化成熟有联系,可能以胰腺干细胞巢的形式分布于胰腺组织中。

Objective： Exploring the relationship between PDG and regeneration of pancreatic ductal epithelium under the stimulation of inflammation. Methods： We evaluate whether the mouse model of acute and chronic pancreatitis was established successfully by pathological score; Histopathological changes of PDG and ducts in pancreatic tissue were observed by histopathological methods; The expression of Pdx1,Sox9 and Ngn3 in PDG and pancreatic ductal epithelial cells were observed by immunohistochemistry,and the positive expression rate of markers was evaluated by HIS score. Results： Mouse models of acute and chronic pancreatitis were successfully established,and the pathological scores of group AP and group CP were 3. 33 ± 0. 52 and 8. 50 ± 0. 55. There were significant differences compared with the control group with a statistical significance; In the models of chronic pancreatitis,proliferation of PDG were obvious,and the expression of both Pdx1,Sox9 and Ngn3 was positive,The HIS scores were1. 966 7 ± 0. 233 8,0. 700 0 ± 0. 209 8,0. 900 0 ± 0. 209 8 respectively. There was a significant difference compared with the control group with a statistical significance. Conclusion： PDG is related with the regeneration of pancreatic ductal epithelium and the differentiation and maturation of adult pancreas. PDG may be distributed in pancreatic tissue in the form of pancreatic stem cell nests.