CYP2D6基因多态与氯氮平血药浓度的关系

A study on the relationship between CYP2D6 gene polymorphisms and plasma concentration of clozapine

目的研究细胞色素酶CYP2D6基因多态性与氯氮平血药浓度的关系。方法对88例服用氯氮平治疗的汉族精神分裂症住院患者进行临床资料收集和氯氮平血药浓度测定,并采用荧光原位杂交技术对CYP2D6基因3个单核苷酸多态性(SNPs)rs16947、rs1065852、rs5030865进行基因分型。结果 rs16947(2850C>T)3种基因型(CC、CT、TT)和rs1065852(100C>T)3种基因型(CC、CT、TT)患者间剂量矫正氯氮平血药浓度比较差异无统计学意义(P>0.05),所有患者rs5030865(1758G>A)基因分型都为GG型。64例CYP2D6强代谢者与24例CYP2D6中代谢者间的剂量矫正氯氮平血药浓度比较差异无统计学意义(P>0.05)。结论 CYP2D6基因的rs16947、rs1065852和rs5030865多态不对氯氮平血药浓度产生影响。

Objective To explore the relationship between CYP2D6 gene polymorphisms and plasma concentration of clozapine. Methods 88 Han inpatients with schizophrenia treated with clozapine were selected and clinical data and plasma concentration of clozapine were obtained. Three single nucleotide polymorphisms（ SNPs） of CYP2D6 genes（ rs16947、rs1065852、rs5030865） were genotyped by fluorescence in situ hybridization（ FISH）. Results There were no significant differences of plasma concentration of dose-adjusted clozapine in three genotypes（ CC、CT、TT） of rs16947（ 2850 C T,P〉0. 05） and in three genotypes（ CC、CT、TT） of rs1065852（ 100 C T,P〉0. 05）. All patients＇ genotypes of rs5030865（ 1758G A） were GG. There was no significant difference of plasma concentration of dose-adjusted clozapine between the 64 CYP2D6 extensive metabolizers and 24 CYP2D6 intermediate motabolizers（ P〉0. 05）. Conclusion Three SNPs of CYP2D6 genes（ rs16947、rs1065852、rs5030865） have no influence on plasma concentration of clozapine.