凤尾草中黄酮提取物对小鼠光老化损伤皮肤的修复作用

Effects of flavonoids extract from Pteris multifida Poir on photoaged skin of mice

目的探讨凤尾草中黄酮提取物对因紫外线照射所致小鼠皮肤光老化的具体修复作用,为该植物的药用提供理论基础。方法选取经过紫外线照射所致光老化的40只损伤模型小鼠,按照性别匹配分为A组(不做处理)、B组(生理盐水涂抹)、C组(生理盐水+维生素C)、D组(生理盐水+黄酮芦丁提取物),每组10只。随访观察10 d,比较四组小鼠皮肤粗糙度(SER)、表面鳞屑(SESC)、皱纹评估(SEW)、愈合面积百分比、微血管密度(MVD)、皮肤愈合时间、结痂时间、绒毛生成时间的差异。结果第7天和第10天,A、B、C组SER、SESC、SEW均显著高于D组(均P<0.05),愈合面积百分比、组织MVD均显著低于D组(均P<0.05)。A、B、C组小鼠皮肤愈合时间、皮肤结痂时间、绒毛生成时间均显著短于D组(均P<0.05),且A组小鼠的各项指标测定结果最大,而D组小鼠的各项指标测定结果最小;皮肤组织病理学检查结果显示,A组小鼠皮肤胶原纤维呈现出无规则断裂状,B组小鼠皮肤真皮层的空洞进行性扩大,C组小鼠皮肤的表皮相对完整,D组小鼠皮肤的表皮恢复情况较好,胶原纤维量也高于其他组别。结论生物类黄酮芦丁物质有助于皮肤光老化损伤的修复,表现在皮肤外观性状和内部组织结构上,其疗效优于维生素C,能显著提升皮肤愈合时间。

Objective To investigate the specific repairing effects of flavonoids extract from Pteris multifida Poir on photoaged skin of mice caused by ultraviolet irradiation, and to provide a theoretical basis for the medicinal use of this plant. Methods Forty damaged model mice with photoaging caused by ultraviolet irradiation were selected, according to the gender, they were divided into group A （no treatment）, group B （smeared normal saline）, group C （smeared normal saline ＋ vitamin C）, group D （smeared normal saline ＋ flavonoids rutin extract）, with 10 mice in each group. Follow-up observation of 10 days, the differences of surface evaluation of roughness （SER）, surface evaluation of scales （SESC）, sur- face evaluation of wrinkles （SEW）, percentage of healed area, microvessel density （MVD）, skin healing time, scab time and fluff generated time among the four groups were compared. Results In the 7th and 10th days, the SER, SESC, and SEW in group A, B, and C were all significantly higher than those of group D （all P 〈 0.05）, the percentage of healed area and MVD were significantly lower than those of group D （all P 〈 0.05）. The skin healing time, scab time and fluff gen- erated time in group A, B, C were significantly shorter than those of group D （all P 〈 0.05）, and the results of each index in group A were maximum, and the results of each index in group D were minimum. Skin histopathologic examination showed that, the skin collagen fibre in group A showed no regular fracture, the emptiness of the dermis in group B was enlarged gradually, the epidermis of group C was relatively intact, the epidermis of group D had better recovery conditions and collagen fibers were also more than other groups. Conclusion Biological flavonoids rutin substances contribute to the repair of photoaged skin, reflected in the skin appearance and internal organizational structure, the efficacy is better than vitamin C, which can greatly enhance the skin healing time.